Rabbit anti-Human ATP6V1F Polyclonal Antibody | anti-ATP6V1F antibody

ATP6V1F, CT (ATP6V1F, ATP6S14, VATF, V-type proton ATPase subunit F, V-ATPase 14kD subunit, Vacuolar proton pump subunit F)

Cat. Num. AAA642625

• Gene Names: ATP6V1F; VATF; Vma7; ATP6S14
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: ATP6V1F; Polyclonal Antibody; CT (ATP6V1F; ATP6S14; VATF; V-type proton ATPase subunit F; V-ATPase 14kD subunit; Vacuolar proton pump subunit F); Anti -ATP6V1F; anti-ATP6V1F antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-ATP6V1F antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in Western Blot, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500
• Immunogen: ATP6V1F antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 81-111 amino acids from the C-terminal region of human ATP6V1F.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(ATP6V1F Antibody (C-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the ATP6V1F antibody detected the ATP6V1F protein (arrow).)

Related Product Information for anti-ATP6V1F antibody

This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is the V1 domain F subunit protein.

Product Categories/Family for anti-ATP6V1F antibody

Antibodies; Abs to Enzymes; ATPase

NCBI and Uniprot Product Information

• NCBI GI #: 48145909
• NCBI GeneID: 9296
• UniProt Accession #: Q16864; Q6IBA8; C9J2K4
• Molecular Weight: 13,370 Da
• NCBI Official Full Name: ATP6V1F
• NCBI Official Synonym Full Names: ATPase, H+ transporting, lysosomal 14kDa, V1 subunit F
• NCBI Official Symbol: ATP6V1F
• NCBI Official Synonym Symbols: VATF; Vma7; ATP6S14
• NCBI Protein Information: V-type proton ATPase subunit F; V-ATPase F subunit; V-ATPase subunit F; ATPase, vacuolar, 14 kD; V-ATPase 14 kDa subunit; vacuolar proton pump F subunit; vacuolar proton pump subunit F; vacuolar ATP synthase subunit F; adenosinetriphosphatase 14k chain; H(+)-transporting two-sector ATPase, 14kD subunit
• UniProt Protein Name: V-type proton ATPase subunit F
• UniProt Gene Name: ATP6V1F
• UniProt Synonym Gene Names: ATP6S14; VATF; V-ATPase subunit F
• UniProt Entry Name: VATF_HUMAN

NCBI Description

This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is the V1 domain F subunit protein. [provided by RefSeq, Jul 2008]

Uniprot Description

ATP6V1F: Subunit of the peripheral V1 complex of vacuolar ATPase essential for assembly or catalytic function. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. Belongs to the V-ATPase F subunit family.

Protein type: Hydrolase

Chromosomal Location of Human Ortholog: 7q32

Cellular Component: membrane; vacuolar proton-transporting V-type ATPase complex; proton-transporting two-sector ATPase complex; cytosol

Molecular Function: protein binding; ATPase activity, uncoupled; hydrogen ion transmembrane transporter activity; hydrogen ion transporting ATPase activity, rotational mechanism

Biological Process: interaction with host; proton transport; metabolic process; cellular iron ion homeostasis; ATP hydrolysis coupled proton transport; insulin receptor signaling pathway; transferrin transport; transmembrane transport