Activating Transcription Factor 6 (ATF6) Recombinant Protein | ATF6 recombinant protein

Recombinant Activating Transcription Factor 6 (ATF6)

Cat. Num. AAA2018452

• Gene Names: ATF6; ATF6A
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Activating Transcription Factor 6 (ATF6); Recombinant Activating Transcription Factor 6 (ATF6); ATF6 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- AKEAQDTS DGIIQKNSYR YDHSVSNDKA LMVLTEEPLL YIPPPPCQPL INTTESLRLN HELRGWVHRH EVERTKSRRM TNNQQKTRIL QGALEQGSNS QLMAVQYTET TSSISRNSGS ELQVYYASPR SYQDFFEAIR RRGDTFYVVS FRRDHLLLPA TTHNKTTRPK MSIVLPAINI NENVINGQDY EVMMQIDCQVMDTRILHIKS SSVPPYLRDQ QRNQTN
• Sequence Length: 670

• Applicable Applications for ATF6 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Homo sapiens (Human)
• Expression System: Prokaryotic expression
• Residues: Ala423~Asn646 (Accession # P18850) with two N-terminal Tags, His-tag and T7-tag
• Subcellular Location: Endoplasmic reticulum membrane; Single-pass type II membrane protein. Nucleus
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Predicted Isoelectric Point: 9.1
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 56786157
• NCBI GeneID: 22926
• NCBI Accession #: NP_031374.2
• NCBI GenBank Nucleotide #: NM_007348.3
• UniProt Accession #: P18850; O15139; Q5VW62; Q6IPB5; Q9UEC9
• Molecular Weight: 29.7kDa
• NCBI Official Full Name: cyclic AMP-dependent transcription factor ATF-6 alpha
• NCBI Official Synonym Full Names: activating transcription factor 6
• NCBI Official Symbol: ATF6
• NCBI Official Synonym Symbols: ATF6A
• NCBI Protein Information: cyclic AMP-dependent transcription factor ATF-6 alpha; cAMP-dependent transcription factor ATF-6 alpha
• UniProt Protein Name: Cyclic AMP-dependent transcription factor ATF-6 alpha
• Protein Family: Cyclic AMP-dependent transcription factor
• UniProt Gene Name: ATF6
• UniProt Synonym Gene Names: cAMP-dependent transcription factor ATF-6 alpha; ATF6-alpha
• UniProt Entry Name: ATF6A_HUMAN

NCBI Description

This gene encodes a transcription factor that activates target genes for the unfolded protein response (UPR) during endoplasmic reticulum (ER) stress. Although it is a transcription factor, this protein is unusual in that it is synthesized as a transmembrane protein that is embedded in the ER. It functions as an ER stress sensor/transducer, and following ER stress-induced proteolysis, it functions as a nuclear transcription factor via a cis-acting ER stress response element (ERSE) that is present in the promoters of genes encoding ER chaperones. This protein has been identified as a survival factor for quiescent but not proliferative squamous carcinoma cells. There have been conflicting reports about the association of polymorphisms in this gene with diabetes in different populations, but another polymorphism has been associated with increased plasma cholesterol levels. This gene is also thought to be a potential therapeutic target for cystic fibrosis. [provided by RefSeq, Aug 2011]

Uniprot Description

ATF6A: Transcription factor that acts during endoplasmic reticulum stress by activating unfolded protein response target genes. Binds DNA on the 5'-CCAC[GA]-3'half of the ER stress response element (ERSE) (5'-CCAAT-N(9)-CCAC[GA]-3') and of ERSE II (5'-ATTGG-N-CCACG-3'). Binding to ERSE requires binding of NF-Y to ERSE. Could also be involved in activation of transcription by the serum response factor. Belongs to the bZIP family. ATF subfamily.

Protein type: Transcription factor; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q23.3

Cellular Component: Golgi membrane; nucleoplasm; Golgi apparatus; endoplasmic reticulum membrane; membrane; endoplasmic reticulum; integral to endoplasmic reticulum membrane; nuclear envelope; nucleus

Molecular Function: protein binding; protein heterodimerization activity; transcription coactivator activity; transcription factor activity

Biological Process: regulation of transcription from RNA polymerase II promoter; unfolded protein response, activation of signaling protein activity; cellular protein metabolic process; protein folding; transcription, DNA-dependent; unfolded protein response; positive regulation of transcription of target genes involved in unfolded protein response; response to stress; signal transduction

Disease: Achromatopsia 7