Rabbit anti-Human MKNK1 Polyclonal Antibody | anti-MKNK1 antibody

MKNK1 (MAP Kinase-interacting Serine/Threonine-protein Kinase 1, MAP Kinase Signal-integrating Kinase 1, MAPK Signal-integrating Kinase 1) (AP)

Cat. Num. AAA6385511

• Gene Names: MKNK1; MNK1
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: MKNK1; Polyclonal Antibody; MKNK1 (MAP Kinase-interacting Serine/Threonine-protein Kinase 1; MAP Kinase Signal-integrating Kinase 1; MAPK Signal-integrating Kinase 1) (AP); anti-MKNK1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MKNK1.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-MKNK1 antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human MKNK1, aa1-465 (NP_003675.2).
• Immunogen Sequence: MVSSQKLEKPIEMGSSEPLPIADGDRRRKKKRRGRATDSLPGKFEDMYKLTSELLGEGAYAKVQGAVSLQNGKEYAVKIIEKQAGHSRSRVFREVETLYQCQGNKNILELIEFFEDDTRFYLVFEKLQGGSILAHIQKQKHFNEREASRVVRDVAAALDFLHTKDKVSLCHLGWSAMAPSGLTAAPTSLGSSDPPTSASQVAGTTGIAHRDLKPENILCESPEKVSPVKICDFDLGSGMKLNNSCTPITTPELTTPCGSAEYMAPEVVEVFTDQATFYDKRCDLWSLGVVLYIMLSGYPPFVGHCGADCGWDRGEVCRVCQNKLFESIQEGKYEFPDKDWAHISSEAKDLISKLLVRDAKQRLSAAQVLQHPWVQGQAPEKGLPTPQVLQRNSSTMDLTLFAAEAIALNRQLSQHEENELAEEPEALADGLCSMKLSPPCKSRLARRRALAQAGRGEDRSPPTAL
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(MKNK1 rabbit polyclonal antibody. Western Blot analysis of MKNK1 expression in human kidney.)

Western Blot (WB)

(Western Blot analysis of MKNK1 expression in transfected 293T cell line by MKNK1 polyclonal antibody. Lane 1: MKNK1 transfected lysate (51.3kD). Lane 2: Non-transfected lysate.)

Testing Data

(Proximity Ligation Analysis (PLA) of protein-protein interactions between MKNK1 and PAK2 HeLa cells were stained with MKNK1 rabbit purified polyclonal 1:1200 and PAK2 mouse monoclonal antibody 1:50. Signals were detected by 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)

Related Product Information for anti-MKNK1 antibody

MNK1 is an MNK protein kinase that is activated in response to mitogens/insulin by ERK1 and ERK2, or in response to stress by activated p38. MNK1 phosphorylates eIF4E to enhance the translation rates of cap-containing mRNAs.

Product Categories/Family for anti-MKNK1 antibody

Antibodies; Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 21361101
• NCBI GeneID: 8569
• NCBI Accession #: NP_003675.2
• NCBI GenBank Nucleotide #: NM_003684.3
• UniProt Accession #: Q9BUB5; O00312; Q5TC06; Q5TC07; Q6V0N6; D3DQ20; D3DQ21
• Molecular Weight: 39,104 Da
• NCBI Official Full Name: MAP kinase-interacting serine/threonine-protein kinase 1 isoform 1
• NCBI Official Synonym Full Names: MAP kinase interacting serine/threonine kinase 1
• NCBI Official Symbol: MKNK1
• NCBI Official Synonym Symbols: MNK1
• NCBI Protein Information: MAP kinase-interacting serine/threonine-protein kinase 1; MAPK signal-integrating kinase 1; MAP kinase signal-integrating kinase 1
• UniProt Protein Name: MAP kinase-interacting serine/threonine-protein kinase 1
• Protein Family: MAP kinase-interacting serine/threonine-protein kinase
• UniProt Gene Name: MKNK1
• UniProt Synonym Gene Names: MNK1; MAPK signal-integrating kinase 1; Mnk1
• UniProt Entry Name: MKNK1_HUMAN

NCBI Description

This gene encodes a Ser/Thr protein kinase that interacts with, and is activated by ERK1 and p38 mitogen-activated protein kinases, and thus may play a role in the response to environmental stress and cytokines. This kinase may also regulate transcription by phosphorylating eIF4E via interaction with the C-terminal region of eIF4G. Alternatively spliced transcript variants have been noted for this gene. [provided by RefSeq, Jan 2012]

Uniprot Description

Mnk1: a CAM kinase of the MAPKKAPK family that can regulate translation. Following activation by MAP kinases, Mnk1 phosphorylates eIF4E at Ser209, increasing its affinity for the 7-methylguanosine-containing mRNA cap. Two MAPK phosphorylation sites in mouse Mnk1 (Thr197 and Thr202) are essential for its kinase activity. Three alternatively spliced human isoforms have been described.

Protein type: Protein kinase, CAMK; EC 2.7.11.1; Translation; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); CAMK group; MAPKAPK family; MNK subfamily

Chromosomal Location of Human Ortholog: 1p33

Cellular Component: nucleus; cytosol

Molecular Function: protein serine/threonine kinase activity; protein binding; ATP binding

Biological Process: peptidyl-serine phosphorylation; fibroblast growth factor receptor signaling pathway; protein amino acid phosphorylation; response to salt stress; regulation of translational initiation