Mouse Myogenin Monoclonal Antibody | anti-MYOG antibody

Myogenin (Skeletal Muscle Marker) Mouse Monoclonal Antibody

Cat. Num. AAA438443

• Gene Names: MYOG; MYF4; myf-4; bHLHc3
• Reactivity: Human, Mouse, Rat, Cat, Pig
• Applications: Immunohistochemistry
• Synonyms: Myogenin; Monoclonal Antibody; Myogenin (Skeletal Muscle Marker) Mouse Monoclonal Antibody; bHLHc3; cb553; Class C basic helix-loop-helix protein 3; Myf-4; MYF4; MYOG; Myogenic factor 4; Myogenin myogenin factor 4; anti-MYOG antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat, Cat, Pig
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: SPM144
• Specificity: Myogenin is a member of the MyoD family of myogenic basic helix-loop-helix (bHLH) transcription factors that also includes MyoD, Myf-5, and MRF4 (also known as herculinor Myf-6). MyoD family members are expressed exclusively in skeletal muscle and play a key role in activating myogenesis by binding to enhancer sequences of muscle-specific genes. The regulatory domain of MyoD is approximately 70 amino acids in length and includes both a basic DNA binding motif and a bHLH dimerization motif. MyoD family members share about 80% amino acid homology in their bHLH motifs. Anti-myogenin labels the nuclei of myoblasts in developing muscle tissue, and is expressed in tumor cell nuclei of rhabdomyosarcoma and some leiomyosarcomas. Positive nuclear staining may occur in Wilms tumor.
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 224

• Applicable Applications for anti-MYOG antibody: Immunohistochemistry (IHC) Formalin
• Application Notes: Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 mins.) Optimal dilution for a specific application should be determined.
• Cellular Localization: Nuclear
• Immunogen: Human myogenin recombinant protein
• Hu-Chromosome Location: 1q32.1
• Positive Control: Rh-30 cells. Skeletal muscle or rhabdomyosarcoma.
• Preparation and Storage: Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human Rhabdomyosarcoma stained with Myogenin Monoclonal Antibody (SPM144))

References

Wang NP et. al. Am J Pathol 1995, 147:1799-1810

NCBI and Uniprot Product Information

• NCBI GI #: 18765727
• NCBI GeneID: 4656
• NCBI Accession #: NP_002470.2
• NCBI GenBank Nucleotide #: NM_002479.5
• UniProt Accession #: P15173; Q53XW6
• Molecular Weight: 34kDa
• NCBI Official Full Name: myogenin
• NCBI Official Synonym Full Names: myogenin (myogenic factor 4)
• NCBI Official Symbol: MYOG
• NCBI Official Synonym Symbols: MYF4; myf-4; bHLHc3
• NCBI Protein Information: myogenin
• UniProt Protein Name: Myogenin
• Protein Family: Myosin
• UniProt Gene Name: MYOG
• UniProt Synonym Gene Names: BHLHC3; MYF4; bHLHc3; Myf-4
• UniProt Entry Name: MYOG_HUMAN

NCBI Description

Myogenin is a muscle-specific transcription factor that can induce myogenesis in a variety of cell types in tissue culture. It is a member of a large family of proteins related by sequence homology, the helix-loop-helix (HLH) proteins. It is essential for the development of functional skeletal muscle. [provided by RefSeq, Jul 2008]

Uniprot Description

myogenin: Involved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Probable sequence specific DNA-binding protein.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 1q31-q41

Cellular Component: nucleoplasm; nucleus; transcription factor complex

Molecular Function: chromatin DNA binding; protein binding; protein heterodimerization activity; RNA polymerase II transcription factor activity, enhancer binding; sequence-specific DNA binding; transcription factor activity

Biological Process: mRNA transcription from RNA polymerase II promoter; muscle cell fate commitment; negative regulation of cell proliferation; positive regulation of muscle atrophy; positive regulation of muscle cell differentiation; positive regulation of myoblast differentiation; positive regulation of skeletal muscle fiber development; positive regulation of transcription from RNA polymerase II promoter; regulation of satellite cell proliferation; response to denervation involved in regulation of muscle adaptation; response to electrical stimulus involved in regulation of muscle adaptation; response to muscle activity involved in regulation of muscle adaptation; skeletal muscle development; striated muscle atrophy