Paired Immunoglobulin-Like Type 2 Receptor beta Recombinant Protein | PILRB recombinant protein

Paired Immunoglobulin-Like Type 2 Receptor beta, Recombinant, Mouse (PILRb)

Cat. Num. AAA638028

• Gene Names: PILRB; FDFACT1; FDFACT2
• Reactivity: Mouse
• Applications: Western Blot
• Purity: Highly Purified; 95%, as determined by SDS-PAGE and visualized by silver stain. Endotoxin 1EU.
• Synonyms: Paired Immunoglobulin-Like Type 2 Receptor beta; Recombinant; Mouse (PILRb); PILRB recombinant protein

• Reactivity: Mouse
• Purity/Purification: Highly Purified; 95%, as determined by SDS-PAGE and visualized by silver stain. Endotoxin 1EU.
• Form/Format: Supplied as a lyophilized powder in PBS. Reconstitute with sterile PBS to 0.1mg/ml.

• Applicable Applications for PILRB recombinant protein: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 19.6kD migrates as an ~ 39-44kD protein in SDS-PAGE under reducing conditions.
• Preparation and Storage: Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile PBS and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for PILRB recombinant protein

PILR-Beta (paired immunoglobulin-like type 2 receptor-beta) is one of two members of a small family of immunoregulatory Ig-superfamily receptors (1, 2). It is a counterpart to PILR-alpha and it likely arose through PILR-alpha gene duplication and rearrangement (1). The PILRs represent one of many pairs of Ig-like domain-containing receptors that participate in immune regulation. PILR-Beta and -alpha should not be confused with the similarly named PIRs (also paired immunoglobulin-like receptors), or the functionally-related SIRP and ILT/LILR/CD85/LIR family of receptors (2). While PIRs, ILTs and SIRPs contain three to six Ig-like domains in their extracellular region, PILR-Beta and -alpha show only one Ig-like region in their extracellular domain (ECD) (1, 2). Mouse PILR-Beta is a 196 amino acid (aa) type I transmembrane (TM) protein (3). It contains a 167 aa ECD, a 21 aa TM segment, and a short, eight aa cytoplasmic region. The ECD shows a V-type Ig-like domain (aa 39-135), while the TM segment contains a positively-charged Lys at position # 202. This Lys is known to interact with the transmembrane signaling adaptor protein DAP12, making PILR-Beta an activating receptor. Activation of PILR-Beta through CD99 ligation induces NK cell cytotoxicity and dendritic cell secretion of NO and TNF-alpha (3). Mouse PILR-Beta is found on NK cells, neutrophils, macrophages, and monocyte-derived dendritic cells (3). Mouse PILR-Beta ECD is 44% and 75% aa identical to human and rat PILR-Beta ECD, respectively; it is 75% aa identical to the ECD of mouse PILR-alpha (3). Evidence suggests that mouse PILR-Beta will not be active in a human system (3). Potential isoforms of PILR-Beta have been reported. One shows an alternate start site at Met14, a second shows a 21 aa substitution for the C-terminal 67 aa, and a third exhibits multiple polymorphisms for an overall aa identity of 86% (4, 5, 6).

Product Categories/Family for PILRB recombinant protein

Molecular Biology; MB-Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 30179911
• NCBI GeneID: 29990
• NCBI Accession #: NP_839956.1
• NCBI GenBank Nucleotide #: NM_178238.3
• UniProt Accession #: Q9UKJ0; Q69YF9; Q9HBS0
• Molecular Weight: 19.6kD
• NCBI Official Full Name: paired immunoglobulin-like type 2 receptor beta
• NCBI Official Synonym Full Names: paired immunoglobin-like type 2 receptor beta
• NCBI Official Symbol: PILRB
• NCBI Official Synonym Symbols: FDFACT1; FDFACT2
• NCBI Protein Information: paired immunoglobulin-like type 2 receptor beta; activating receptor PILRbeta; cell surface receptor FDFACT; activating receptor PILR-beta; cell surface receptor FDFACT1; cell surface receptor FDFACT2; paired immunoglobin-like receptor beta; paired immunoglobulin-like receptor beta
• UniProt Protein Name: Paired immunoglobulin-like type 2 receptor beta
• Protein Family: Paired immunoglobulin-like type 2 receptor
• UniProt Gene Name: PILRB
• UniProt Synonym Gene Names: FDFACT
• UniProt Entry Name: PILRB_HUMAN

NCBI Description

The paired immunoglobin-like type 2 receptors consist of highly related activating and inhibitory receptors that are involved in the regulation of many aspects of the immune system. The paired immunoglobulin-like receptor genes are located in a tandem head-to-tail orientation on chromosome 7. This gene encodes the activating member of the receptor pair and contains a truncated cytoplasmic tail relative to its inhibitory counterpart (PILRA), that has a long cytoplasmic tail with immunoreceptor tyrosine-based inhibitory (ITIM) motifs. This gene is thought to have arisen from a duplication of the inhibitory PILRA gene and evolved to acquire its activating function. [provided by RefSeq, Jun 2013]

Uniprot Description

PILRB: Paired receptors consist of highly related activating and inhibitory receptors and are widely involved in the regulation of the immune system. PILRB is thought to act as a cellular signaling activating receptor that associates with ITAM-bearing adapter molecules on the cell surface. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Immunoglobulin superfamily; Membrane protein, integral

Chromosomal Location of Human Ortholog: 7q22.1

Cellular Component: integral to plasma membrane

Molecular Function: protein binding

Biological Process: transmembrane receptor protein tyrosine kinase activation (dimerization); transmembrane receptor protein tyrosine kinase signaling pathway