Immunoglobulin Associated Beta (Igb) Recombinant Protein | Igb recombinant protein

Recombinant Immunoglobulin Associated Beta (Igb)

Cat. Num. AAA2018252

• Gene Names: Cd79b; B29; Igb; Igbeta; Ig-beta
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Immunoglobulin Associated Beta (Igb); Recombinant Immunoglobulin Associated Beta (Igb); Igb recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- VPAMT SSDLPLNFQG SPCSQIWQHP RFAAKKRSSM VKFHCYTNHS GALTWFRKRG SQQPQELVSE EGRIVQTQNG SVYTLTIQNI QYEDNGIYFC KQKCDSANHN VTDSCGTELL VLGFSTLDQL KRRNTLKDGI ILIQTLLIIL FIIVPIFLLL DKDDGKAGME EDHTYEGLNI DQTATYEDIVTLRTGEVKWS VGEHP
• Sequence Length: 228

• Applicable Applications for Igb recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Val26~Pro225 (Accession # P15530) with two N-terminal Tags, His-tag and T7-tag
• Subcellular Location: Cell membrane; Single-pass type I membrane protein
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Predicted Isoelectric Point: 6.5
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 6680375
• NCBI GeneID: 15985
• NCBI Accession #: NP_032365.1
• NCBI GenBank Nucleotide #: NM_008339.2
• UniProt Accession #: P15530; Q4FJP4
• Molecular Weight: 26.4kDa
• NCBI Official Full Name: B-cell antigen receptor complex-associated protein beta chain
• NCBI Official Synonym Full Names: CD79B antigen
• NCBI Official Symbol: Cd79b
• NCBI Official Synonym Symbols: B29; Igb; Igbeta; Ig-beta
• NCBI Protein Information: B-cell antigen receptor complex-associated protein beta chain; immunoglobulin-associated beta; B-cell-specific glycoprotein B29; immunoglobulin-associated B29 protein
• UniProt Protein Name: B-cell antigen receptor complex-associated protein beta chain
• UniProt Gene Name: Cd79b
• UniProt Synonym Gene Names: Igb
• UniProt Entry Name: CD79B_MOUSE

NCBI Description

The B lymphocyte antigen receptor is a multimeric complex that includes the antigen-specific component, surface immunoglobulin (Ig). Surface Ig non-covalently associates with two other proteins, Ig-alpha and Ig-beta, which are necessary for expression and function of the B-cell antigen receptor. This gene encodes the Ig-beta protein of the B-cell antigen component. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Sep 2015]

Uniprot Description

Ig-beta: Required in cooperation with CD79A for initiation of the signal transduction cascade activated by the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. Enhances phosphorylation of CD79A, possibly by recruiting kinases which phosphorylate CD79A or by recruiting proteins which bind to CD79A and protect it from dephosphorylation. Defects in CD79B are the cause of agammaglobulinemia type 6 (AGM6). It is a primary immunodeficiency characterized by profoundly low or absent serum antibodies and low or absent circulating B-cells due to an early block of B-cell development. Affected individuals develop severe infections in the first years of life. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, misc.

Cellular Component: nucleoplasm; Golgi apparatus; membrane; cytoplasm; plasma membrane; integral to membrane; B cell receptor complex; external side of plasma membrane

Molecular Function: protein binding; transmembrane receptor activity

Biological Process: B cell receptor signaling pathway; cell surface receptor linked signal transduction; immune system process