Leukotriene A4 Hydrolase Active Protein | LTA4H active protein

Leukotriene A4 Hydrolase, Recombinant, Human (LTA4H)

Cat. Num. AAA637847

• Purity: Highly Purified; 95%, as determined by SDS-PAGE and visualized by silver stain.
• Synonyms: Leukotriene A4 Hydrolase; Recombinant; Human (LTA4H); LTA4H active protein

• Purity/Purification: Highly Purified; 95%, as determined by SDS-PAGE and visualized by silver stain.
• Form/Format: Supplied as a liquid in 12.5mM Tris, 75mM sodium chloride, 50% glycerol, pH 8.0.

• Activity: Measured by its ability to cleave a fluorogenic peptide substrate, R-AMC. See Activity Assay Protocol on next page for details. The specific activity, measured with 100uM substrate and 0.5ug rhLTA4H in 100ul of 50mM Tris, 150mM NaCl, 10mM CaCl 2, pH 7.5 at RT, is > 40 pmoles/min/ug.
• Endotoxin Level: 1EU/ug of the protein as determined by the LAL method.
• Preparation and Storage: This protein can be aliquoted and stored under sterile conditions at -20 degree C in a manual defrost freezer for up to six months without a significant loss of activity.

Related Product Information for LTA4H active protein

Leukotrienes are a family of lipid mediators important in a variety of allergic and inflammatory reactions. Synthesized by leukocytes, these molecules are divided into two classes, the spasmogenic cysteinyl leukotrienes and LTB4, a classical chemoattractant (1). Encoded by the LTA4H gene, leukotriene A4 hydrolase catalyzes the conversion of unstable epoxide LTA4 to LTB4, which is the final and committed step in LTB4 biosynthesis. As a bifunctional zinc metalloenzyme, LTA4H also acts as an arginyl aminopeptidase (2). LTA4H is a drug target for anti-inflammation, and for cancer prevention and therapy (1,3). The mature chain of human LTA4H consists of 610 amino acids (residues 2 - 611). It is highly specific for LTA4, which also covalently modifies and inhibits the enzyme. The aminopeptidase activity is enhanced by monovalent anions (1). rhLTA4H corresponds to the mature chain, and is characterized by its arginyl aminopeptidase activity.

Product Categories/Family for LTA4H active protein

Molecular Biology; MB-Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 976396
• NCBI GeneID: 4048
• UniProt Accession #: P09960; Q6IAT6; Q9UCT7; B4DNQ9; F8VV40
• Molecular Weight: The purified rhLTA4H has a N-terminal sequence of P 2 EIVDTCSLA. The 620 amino acid residue rhLTA4H predicts a molecular mass of 70kD and migrates with an apparent molecular mass of approximately 66kD in SDS-PAGE under reducing conditions.
• NCBI Official Full Name: leukotriene A4 hydrolase
• NCBI Official Synonym Full Names: leukotriene A4 hydrolase
• NCBI Official Symbol: LTA4H
• NCBI Protein Information: leukotriene A-4 hydrolase; LTA-4 hydrolase
• UniProt Protein Name: Leukotriene A-4 hydrolase
• Protein Family: Leukotriene A-4 hydrolase
• UniProt Gene Name: LTA4H
• UniProt Synonym Gene Names: LTA4; LTA-4 hydrolase
• UniProt Entry Name: LKHA4_HUMAN

NCBI Description

The protein encoded by this gene is an enzyme that contains both hydrolase and aminopeptidase activities. The hydrolase activity is used in the final step of the biosynthesis of leukotriene B4, a proinflammatory mediator. The aminopeptidase activity has been shown to degrade proline-glycine-proline (PGP), a neutrophil chemoattractant and biomarker for chronic obstructive pulmonary disease (COPD). Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015]

Uniprot Description

LTA4H: Epoxide hydrolase that catalyzes the final step in the biosynthesis of the proinflammatory mediator leukotriene B4. Has also aminopeptidase activity. Belongs to the peptidase M1 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.3.2.6; Lipid Metabolism - arachidonic acid; Hydrolase

Chromosomal Location of Human Ortholog: 12q22

Cellular Component: nucleoplasm; cytoplasm; cytosol

Molecular Function: peptidase activity; leukotriene-A4 hydrolase activity; metallopeptidase activity; zinc ion binding; epoxide hydrolase activity; aminopeptidase activity

Biological Process: leukotriene biosynthetic process; peptide catabolic process; arachidonic acid metabolic process; leukotriene metabolic process; proteolysis; inflammatory response