Rabbit anti-Human ERCC6L Polyclonal Antibody | anti-ERCC6L antibody

ERCC6L (DNA Excision Repair Protein ERCC-6-like, ATP-dependent Helicase ERCC6-like, PLK1-interacting Checkpoint Helicase, Tumor Antigen BJ-HCC-15, PICH, FLJ20105, MGC131695) (HRP)

Cat. Num. AAA6377628

• Gene Names: ERCC6L; PICH; RAD26L
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ERCC6L; Polyclonal Antibody; ERCC6L (DNA Excision Repair Protein ERCC-6-like; ATP-dependent Helicase ERCC6-like; PLK1-interacting Checkpoint Helicase; Tumor Antigen BJ-HCC-15; PICH; FLJ20105; MGC131695) (HRP); anti-ERCC6L antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ERCC6L.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
• Sequence Length: 1729

• Applicable Applications for anti-ERCC6L antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human ERCC6L, aa1-419 (AAH08808.1).
• Immunogen Sequence: MEKSFATKNEAVQKETLQEGPKQEALQEDPLESFNYVLSKSTKADIGPNLDQLKDDEILRHCNPWPIISITNESQNAESNVSIIEIADDLSASHSALQDAQASEAKLEEEPSASSPQYACDFNLFLEDSADNRQNFSSQSLEHVEKENSLCGSAPNSRAGFVHSKTCLSWEFSEKDDEPEEVVVKAKIRSKARRIVSDGEDEDDSFKDTSSINPFNTSLFQFSSVKQFDASTPKNDISPPGRFFSSQIPSSVNKSMNSRRSLASRRSLINMVLDHVEDMEERLDDSSEAKGPEDYPEEGVEESSGEASKYTEEDPSGETLSSENKSSWLMTSKPSALAQETSLGAPEPLSGEQLVGSPQDKAAEATNDYETLVKRGKELKECGKIQEALNCLVKALDIKSADPEVMLLTLSLYKQLNNN For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap/
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of ERCC6L expression in transfected 293T cell line by ERCC6L polyclonal antibody. Lane 1: ERCC6L transfected lysate (46.2kD). Lane 2: Non-transfected lysate.)

Related Product Information for anti-ERCC6L antibody

DNA helicase that acts as an essential component of the spindle assembly checkpoint. Contributes to the mitotic checkpoint by recruiting MAD2 to kinetochores and monitoring tension on centromeric chromatin. Acts as a tension sensor that associates with catenated DNA which is stretched under tension until it is resolved during anaphase.

Product Categories/Family for anti-ERCC6L antibody

Antibodies; Cell Cycle Inhibitors

References

1. The relative efficiency of homology-directed repair has distinct effects on proper anaphase chromosome separation. Laulier C, Cheng A, Stark JM.Nucleic Acids Res. 2011 Mar 31.

NCBI and Uniprot Product Information

• NCBI GI #: 33871026
• NCBI GeneID: 54821
• NCBI Official Full Name: Homo sapiens excision repair cross-complementing rodent repair deficiency, complementation group 6-like, mRNA
• NCBI Official Synonym Full Names: ERCC excision repair 6 like, spindle assembly checkpoint helicase
• NCBI Official Symbol: ERCC6L
• NCBI Official Synonym Symbols: PICH; RAD26L
• NCBI Protein Information: DNA excision repair protein ERCC-6-like
• Protein Family: DNA excision repair protein

NCBI Description

This gene encodes a member of the SWItch/Sucrose Non-Fermentable (SWI/SNF2) family of proteins, and contains a SNF2-like ATPase domain and a PICH family domain. One distinguishing feature of this SWI/SNF protein family member is that during interphase, the protein is excluded from the nucleus, and only associates with chromatin after the nuclear envelope has broken down. This protein is a DNA translocase that is thought to bind double-stranded DNA that is exposed to stretching forces, such as those exerted by the mitotic spindle. This protein associates with ribosomal DNA and ultra-fine DNA bridges (UFBs), fine structures that connect sister chromatids during anaphase at some sites such as fragile sites, telomeres and centromeres. This gene is required for the faithful segregation of sister chromatids during mitosis, and the ATPase activity of this protein required for the resolution of UFBs before cytokinesis. [provided by RefSeq, May 2017]