Immunofluorescence (IF)
(MBS9602277 staining Hela cells by IF/ICC. The samples were fixedwith PFA and permeabilized in 0.1% Triton X-100,then blockedin 10% serum for 45 minutes at 25°C. Samples were thenincubated with primary Ab(MBS9602277 1:200) and mouse anti-beta tubulin Ab(MBS9610328 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red)and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L)Ab(Green) were used as the secondary antibody.)
Rabbit anti-Human ZIP2 Polyclonal Antibody | anti-ZIP2 antibody
ZIP2 Antibody
Gene Names
SLC39A2; 6A1; ZIP2; ETI-1; ZIP-2
Reactivity
Human
Applications
ELISA, Immunocytochemistry, Immunofluorescence, Western Blot
Purity
Peptide affinity purification
Synonyms
ZIP2; Polyclonal Antibody; ZIP2 Antibody; hZIP2; 6A1; Eti-1; Eti1; MGC119190; OTTHUMP00000164402; S39A2_HUMAN; SLC39A2; Solute carrier family 39 (zinc transporter) member 2; Solute carrier family 39 member 2; Zinc transporter ZIP2; ZIP 2; ZIP-2; Zrt and Irt like protein 2; Zrt-and Irt-like protein 2; anti-ZIP2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
ZIP2 Antibody detects endogenous levels of total ZIP2.
Purity/Purification
Peptide affinity purification
Form/Format
IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-ZIP2 antibody
WB: 1:1000
IF/ICC: 1:100-1:500
ELISA(peptide): 1:20000-1:40000
* The optimal dilutions should be determined by the end user.
IMPORTANT: For western blot, incubate membrane with diluted primary Abin 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C wi
IF/ICC: 1:100-1:500
ELISA(peptide): 1:20000-1:40000
* The optimal dilutions should be determined by the end user.
IMPORTANT: For western blot, incubate membrane with diluted primary Abin 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C wi
Immunogen
A synthesized peptide derived from human ZIP2, corresponding to a region within N-terminal amino acids.
Preparation and Storage
Store at -20°C.
Immunofluorescence (IF)
(MBS9602277 staining Hela cells by IF/ICC. The samples were fixedwith PFA and permeabilized in 0.1% Triton X-100,then blockedin 10% serum for 45 minutes at 25°C. Samples were thenincubated with primary Ab(MBS9602277 1:200) and mouse anti-beta tubulin Ab(MBS9610328 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red)and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L)Ab(Green) were used as the secondary antibody.)
Immunofluorescence (IF)
(MBS9602277 staining Hela cells by IF/ICC. The samples were fixedwith PFA and permeabilized in 0.1% Triton X-100,then blockedin 10% serum for 45 minutes at 25°C. Samples were thenincubated with primary Ab(MBS9602277 1:200) and mouse anti-beta tubulin Ab(MBS9610328 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red)and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L)Ab(Green) were used as the secondary antibody.)
Western Blot (WB)
(Western blot analysis of ZIP2 using Jurkat whole cell lysates)
Western Blot (WB)
(Western blot analysis of ZIP2 using Jurkat whole cell lysates)
Related Product Information for anti-ZIP2 antibody
Mediates zinc uptake. Zinc uptake may be mediated by a Zn2+-HCO3-symport mechanism and can function in the presence of albumin. May also transport other divalent cations. May be important in contact inhibition of normal epithelial cells and loss of its expression may play a role in tumorigenesis.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
36kDa
NCBI Official Full Name
zinc transporter ZIP2 isoform b
NCBI Official Synonym Full Names
solute carrier family 39 member 2
NCBI Official Symbol
SLC39A2
NCBI Official Synonym Symbols
6A1; ZIP2; ETI-1; ZIP-2
NCBI Protein Information
zinc transporter ZIP2
UniProt Protein Name
Zinc transporter ZIP2
Protein Family
UniProt Gene Name
SLC39A2
UniProt Synonym Gene Names
ZIP2; ZIP-2; hZIP2
NCBI Description
This gene encodes a member of the ZIP family of metal ion transporters. The encoded protein functions as a zinc transporter. Mutations in this gene may be associated with susceptibility to carotid artery disease. Multiple transcript variants have been described. [provided by RefSeq, Mar 2010]
Uniprot Description
Mediates zinc uptake. Zinc uptake may be mediated by a Zn2+-HCO3- symport mechanism and can function in the presence of albumin. May also transport other divalent cations. May be important in contact inhibition of normal epithelial cells and loss of its expression may play a role in tumorigenesis.
Research Articles on ZIP2
Similar Products
Product Notes
The ZIP2 slc39a2 (Catalog #AAA9602277) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ZIP2 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ZIP2 can be used in a range of immunoassay formats including, but not limited to, WB: 1:1000 IF/ICC: 1:100-1:500 ELISA(peptide): 1:20000-1:40000 * The optimal dilutions should be determined by the end user. IMPORTANT: For western blot, incubate membrane with diluted primary Abin 5% w/v milk, 1X TBS, 0.1% Tween®20 at 4°C wi. Researchers should empirically determine the suitability of the ZIP2 slc39a2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ZIP2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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