Western Blot (WB)
(Western Blot detection against Immunogen (72.2kD).)
Mouse anti-Human ERCC6L Monoclonal Antibody | anti-ERCC6L antibody
ERCC6L (DNA Excision Repair Protein ERCC-6-like, ATP-dependent Helicase ERCC6-like, PLK1-interacting Checkpoint Helicase, Tumor Antigen BJ-HCC-15, PICH, FLJ20105, MGC131695) (HRP)
Gene Names
ERCC6L; PICH; RAD26L
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
ERCC6L; Monoclonal Antibody; ERCC6L (DNA Excision Repair Protein ERCC-6-like; ATP-dependent Helicase ERCC6-like; PLK1-interacting Checkpoint Helicase; Tumor Antigen BJ-HCC-15; PICH; FLJ20105; MGC131695) (HRP); anti-ERCC6L antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
3F12-2B10
Specificity
Recognizes human ERCC6L.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Applicable Applications for anti-ERCC6L antibody
ELISA (EIA), Western Blot (WB)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
Full length recombinant corresponding to aa1-420 from human ERCC6L (AAH08808) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
MEKSFATKNEAVQKETLQEGPKQEALQEDPLESFNYVLSKSTKADIGPNLDQLKDDEILRHCNPWPIISITNESQNAESNVSIIEIADDLSASHSALQDAQASEAKLEEEPSASSPQYACDFNLFLEDSADNRQNFSSQSLEHVEKENSLCGSAPNSRAGFVHSKTCLSWEFSEKDDEPEEVVVKAKIRSKARRIVSDGEDEDDSFKDTSSINPFNTSLFQFSSVKQFDASTPKNDISPPGRFFSSQIPSSVNKSMNSRRSLASRRSLINMVLDHVEDMEERLDDSSEAKGPEDYPEEGVEESSGEASKYTEEDPSGETLSSENKSSWLMTSKPSALAQETSLGAPEPLSGEQLVGSPQDKAAEATNDYETLVKRGKELKECGKIQEALNCLVKALDIKSADPEVMLLTLSLYKQLNNN
Conjugate
HRP
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western Blot detection against Immunogen (72.2kD).)
Western Blot (WB)
(Western Blot detection against Immunogen (72.2kD).)
Western Blot (WB)
(Western Blot analysis of ERCC6L expression in transfected 293T cell line by ERCC6L monoclonal antibody. Lane 1: ERCC6L transfected lysate (46.2kD). Lane 2: Non-transfected lysate.)
Western Blot (WB)
(Western Blot analysis of ERCC6L expression in transfected 293T cell line by ERCC6L monoclonal antibody. Lane 1: ERCC6L transfected lysate (46.2kD). Lane 2: Non-transfected lysate.)
Testing Data
(Detection limit for recombinant GST tagged ERCC6L is ~0.03ng/ml as a capture antibody.)
Testing Data
(Detection limit for recombinant GST tagged ERCC6L is ~0.03ng/ml as a capture antibody.)
Related Product Information for anti-ERCC6L antibody
DNA helicase that acts as an essential component of the spindle assembly checkpoint. Contributes to the mitotic checkpoint by recruiting MAD2 to kinetochores and monitoring tension on centromeric chromatin. Acts as a tension sensor that associates with catenated DNA which is stretched under tension until it is resolved during anaphase.
Product Categories/Family for anti-ERCC6L antibody
References
1. PICH and cotargeted Plk1 coordinately maintain prometaphase chromosome arm architecture. Kurasawa Y, Yu-Lee LY.Mol Biol Cell. 2010 Apr;21(7):1188-99. Epub 2010 Feb 3.2.Targeting Plk1 to chromosome arms and regulating chromosome compaction by the PICH ATPase. Leng M, Bessuso D, Jung SY, Wang Y, Qin J.Cell Cycle. 2008 May 15;7(10):1480-9. Epub 2008 Mar 16.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
141,103 Da
NCBI Official Full Name
Homo sapiens excision repair cross-complementing rodent repair deficiency, complementation group 6-like, mRNA
NCBI Official Synonym Full Names
ERCC excision repair 6 like, spindle assembly checkpoint helicase
NCBI Official Symbol
ERCC6L
NCBI Official Synonym Symbols
PICH; RAD26L
NCBI Protein Information
DNA excision repair protein ERCC-6-like
Protein Family
Research Articles on ERCC6L
Similar Products
Product Notes
The ERCC6L (Catalog #AAA6152375) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The ERCC6L (DNA Excision Repair Protein ERCC-6-like, ATP-dependent Helicase ERCC6-like, PLK1-interacting Checkpoint Helicase, Tumor Antigen BJ-HCC-15, PICH, FLJ20105, MGC131695) (HRP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ERCC6L can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the ERCC6L for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ERCC6L, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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