Heat Shock Protein 25 Recombinant Protein | HSP25 recombinant protein
Heat Shock Protein 25, Recombinant, Mouse (HSP25)
Applications
ELISA, Western Blot
Purity
Highly Purified90% as determined by SDS-PAGE analysis.
Synonyms
Heat Shock Protein 25; Recombinant; Mouse (HSP25); HSP25 recombinant protein
Purity/Purification
Highly Purified
90% as determined by SDS-PAGE analysis.
90% as determined by SDS-PAGE analysis.
Form/Format
Supplied as a liquid in 1X Dulbecco's PBS (2.7mM potassium chloride, 1.5mM KH2PO4, 137mM sodium chloride, 8.1mM Na2HPO4).
Applicable Applications for HSP25 recombinant protein
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in Western Blot and ELISA.
Dilution: Western Blot: Dilute in an SDS containing sample buffer. Heat at 100 degree C for 5 minutes prior to use. On a 15-well minigel system, loading 50ng-100ng of protein per lane should be sufficient.
Dilution: Western Blot: Dilute in an SDS containing sample buffer. Heat at 100 degree C for 5 minutes prior to use. On a 15-well minigel system, loading 50ng-100ng of protein per lane should be sufficient.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, aliquot to avoid repeated freezing and thawing and freeze at -70 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 12 months.
Related Product Information for HSP25 recombinant protein
Mouse Hsp25, human Hsp27, and alpha-beta-crystallin are part of a diverse family of small heat shock proteins (sHsps) which are produced in all organisms. The overall homology between the different sHsps is low and they are grouped together based on conserved sequences in the C-terminal half of the protein and short conserved phenylalanine-rich stretches near the N terminus. Mammalian sHsps are expressed constitutively under physiological conditions but stress factors such as heat shock induce a strong up-regulation of protein levels by 10-20-fold to maximum concentrations of 0.1% of the cellular protein. They function as chaperone-like proteins by binding unfolded polypeptides and preventing uncontrolled protein aggregation. sHsps all share the striking feature of forming high molecular weight oligomeric complexes of variable size. The quaternary structure of sHsps is essential for their function and regulation of activity but its basic properties are still rather poorly understood. Data indicates that Hsp25 is a dynamic tetramer of tetramers with a unique ability to refold and reassemble into its active quaternary structure after denaturation (1). Current studies demonstrate that Hsp25 helps facilitate the glutathione-redox cycle by enhancing glutathione utilization and maintaining the cellular glutathione pool in favor of the reduced states (2).
Product Categories/Family for HSP25 recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
heat shock protein 25
NCBI Official Synonym Full Names
heat shock protein 25
NCBI Official Symbol
HSP25
NCBI Protein Information
heat shock protein 25
Research Articles on HSP25
Similar Products
Product Notes
The HSP25 (Catalog #AAA637192) is a Recombinant Protein and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Heat Shock Protein 25 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in Western Blot and ELISA. Dilution: Western Blot: Dilute in an SDS containing sample buffer. Heat at 100 degree C for 5 minutes prior to use. On a 15-well minigel system, loading 50ng-100ng of protein per lane should be sufficient. Researchers should empirically determine the suitability of the HSP25 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Heat Shock Protein 25, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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