Mst1 Active Protein | STK4 active protein

Mst1, Recombinant, Mouse (Hepatocyte Growth Factor-like Protein, Macrophage Stimulatory Protein, MSP, Hgfl)

Cat. Num. AAA637106

• Gene Names: STK4; KRS2; MST1; YSK3; TIIAC
• Purity: Highly Purified; ~95% (SDS-PAGE)
• Synonyms: Mst1; Recombinant; Mouse (Hepatocyte Growth Factor-like Protein; Macrophage Stimulatory Protein; MSP; Hgfl); STK4 active protein

• Host: CHO cells
• Purity/Purification: Highly Purified; ~95% (SDS-PAGE)
• Form/Format: Supplied as a lyophilized powder from PBS, BSA. Reconstitute with PBS, 0.1% human or BSA to 0.1mg/ml.

• Biological Activity: Measured by its binding ability in a functional ELISA. When Recombinant Mouse MSP R/Ron Fc Chimera is coated at 1ug/ml (100ul/well), the concentration of Recombinant Mouse MSP/MST1 that produces 50% of the optimal binding response is found to be ~1-6ng/ml.
• Endotoxin Level: ~1EU/1ug (LAL)
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS, 0.1% HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for STK4 active protein

Macrophage stimulating protein (MSP), also known as HGF-like protein, and scatter factor-2, is a member of the HGF family of growth factors. MSP is secreted as an inactive single chain precursor (pro-MSP) that contains a PAN/APPLE-like domain, four kringle domains, and a peptidase S1 domain which lacks enzymatic activity. Mouse MSP shares 79% and 93% aa sequence identity with human and rat MSP, respectively and 42% aa sequence identity with mouse HGF. Pro-MSP is secreted by hepatocytes under the positive and negative control of CBP in complex with either HNF-4 or RAR, respectively. Circulating pro-MSP is proteolytically cleaved in response to tissue injury to yield biologically active disulfide linked heterodimers consisting of a 45-62kD alpha and a 25-35kD beta chain. Pro-MSP can be activated by MT-SP1, a transmembrane protease that is expressed on macrophages and is upregulated in many cancers. Heterodimeric MSP, as well as the isolated beta chain, binds to MSP R/Ron with high-affinity, although only heterodimeric MSP can induce receptor dimerization and signaling. MSP induces macrophage and keratinocyte proliferation and osteoclast activation. It also inhibits LPS- or IFN-induced iNOS and IL-12 expression by macrophages and prevents apoptosis of epithelial cells separated from the ECM. The substitution of cysteine 672 (in the beta chain) with alanine significantly increases the bioactivity of recombinant human MSP, apparently by limiting incorrect disulfide bond formation between the alpha and beta chains.

Recombinant protein corresponding to aa1-716 from mouse MST1 expressed in Chinese Hamster Ovary cell line, CHO (NP_032269).

Product Categories/Family for STK4 active protein

Growth Factors; Cytokines; Growth Factors-HGF

NCBI and Uniprot Product Information

• NCBI GI #: 1117791
• NCBI GeneID: 6789
• UniProt Accession #: Q13043; Q15802; Q4G156; Q5H982; Q6PD60; Q9BR32; Q9NTZ4; B2RCR8
• Molecular Weight: ~53.7kD & 25kD
• NCBI Official Full Name: MST1
• NCBI Official Synonym Full Names: serine/threonine kinase 4
• NCBI Official Symbol: STK4
• NCBI Official Synonym Symbols: KRS2; MST1; YSK3; TIIAC
• NCBI Protein Information: serine/threonine-protein kinase 4; MST-1; STE20-like kinase MST1; mammalian sterile 20-like 1; kinase responsive to stress 2; mammalian STE20-like protein kinase 1; serine/threonine-protein kinase Krs-2; dJ211D12.2 (serine/threonine kinase 4 (MST1, KRS2))
• UniProt Protein Name: Serine/threonine-protein kinase 4
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: STK4
• UniProt Synonym Gene Names: KRS2; MST1; MST-1; MST1/N; MST1/C
• UniProt Entry Name: STK4_HUMAN

NCBI Description

The protein encoded by this gene is a cytoplasmic kinase that is structurally similar to the yeast Ste20p kinase, which acts upstream of the stress-induced mitogen-activated protein kinase cascade. The encoded protein can phosphorylate myelin basic protein and undergoes autophosphorylation. A caspase-cleaved fragment of the encoded protein has been shown to be capable of phosphorylating histone H2B. The particular phosphorylation catalyzed by this protein has been correlated with apoptosis, and it's possible that this protein induces the chromatin condensation observed in this process. [provided by RefSeq, Jul 2008]

Uniprot Description

MST1: a protein kinase of the STE20 family. Proteolytically activated by caspase during apoptosis. Activated by apoptotic signals as well as other stress conditions. Full activation requires both phosphorylation and caspase-mediated cleavage. Phosphorylation at serine 327 of Mst1, which is close to the caspase-3 recognition site, inhibits caspase-mediated cleavage.

Protein type: Protein kinase, STE; Autophagy; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; STE group; STE20 family; MST subfamily

Chromosomal Location of Human Ortholog: 20q11.2-q13.2

Cellular Component: nucleoplasm; cytoplasm; cytosol; nucleus

Molecular Function: protein dimerization activity; identical protein binding; protein serine/threonine kinase activity; protein serine/threonine kinase activator activity; protein binding; protein homodimerization activity; magnesium ion binding; transcription factor binding; ATP binding; receptor signaling protein serine/threonine kinase activity

Biological Process: central nervous system development; positive regulation of protein binding; positive regulation of apoptosis; apoptosis; protein amino acid autophosphorylation; cell morphogenesis; negative regulation of organ growth; signal transduction; protein amino acid phosphorylation; patterning of blood vessels; keratinocyte differentiation; negative regulation of cell proliferation; peptidyl-serine phosphorylation; positive regulation of fat cell differentiation; neural tube formation

Disease: T-cell Immunodeficiency, Recurrent Infections, And Autoimmunity With Or Without Cardiac Malformations