Rabbit anti-Mouse Stk4 Polyclonal Antibody | anti-STK4 antibody

Stk4, CT (Stk4, Mst1, Serine/threonine-protein kinase 4, Mammalian STE20-like protein kinase 1, STE20-like kinase MST1, Serine/threonine-protein kinase 4 37kDa subunit, Serine/threonine-protein kinase 4 18kDa subunit)

Cat. Num. AAA6013326

• Gene Names: STK4; KRS2; MST1; YSK3; TIIAC
• Reactivity: Mouse
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: Stk4; Polyclonal Antibody; CT (Stk4; Mst1; Serine/threonine-protein kinase 4; Mammalian STE20-like protein kinase 1; STE20-like kinase MST1; Serine/threonine-protein kinase 4 37kDa subunit; Serine/threonine-protein kinase 4 18kDa subunit); Anti -Stk4; anti-STK4 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Mouse
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-STK4 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in Western Blot, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500
• Immunogen: Mouse Stk4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 442-470 amino acids from the C-terminal region of Mouse Stk4.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Mouse Stk4 Antibody (C-term) western blot analysis in mouse cerebellum tissue lysates (35ug/lane).This demonstrates the Stk4 antibody detected the Stk4 protein (arrow).)

Related Product Information for anti-STK4 antibody

Stress-activated, pro-apoptotic kinase which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation. Key component of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. MST1/MST2 are required to repress proliferation of mature hepatocytes, to prevent activation of facultative adult liver stem cells (oval cells), and to inhibit tumor formation. Phosphorylates 'Ser-14' of histone H2B (H2BS14ph) during apoptosis. Phosphorylates FOXO3 upon oxidative stress, which results in its nuclear translocation and cell death initiation.

Product Categories/Family for anti-STK4 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 5454096
• NCBI GeneID: 6789
• NCBI Accession #: NP_006273.1
• NCBI GenBank Nucleotide #: NM_006282.2
• UniProt Accession #: Q13043; Q15802; Q4G156; Q5H982; Q6PD60; Q9BR32; Q9NTZ4; B2RCR8
• Molecular Weight: 55,630 Da
• NCBI Official Full Name: serine/threonine-protein kinase 4
• NCBI Official Synonym Full Names: serine/threonine kinase 4
• NCBI Official Symbol: STK4
• NCBI Official Synonym Symbols: KRS2; MST1; YSK3; TIIAC
• NCBI Protein Information: serine/threonine-protein kinase 4; MST-1; STE20-like kinase MST1; mammalian sterile 20-like 1; kinase responsive to stress 2; mammalian STE20-like protein kinase 1; serine/threonine-protein kinase Krs-2; dJ211D12.2 (serine/threonine kinase 4 (MST1, KRS2))
• UniProt Protein Name: Serine/threonine-protein kinase 4
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: STK4
• UniProt Synonym Gene Names: KRS2; MST1; MST-1; MST1/N; MST1/C
• UniProt Entry Name: STK4_HUMAN

NCBI Description

The protein encoded by this gene is a cytoplasmic kinase that is structurally similar to the yeast Ste20p kinase, which acts upstream of the stress-induced mitogen-activated protein kinase cascade. The encoded protein can phosphorylate myelin basic protein and undergoes autophosphorylation. A caspase-cleaved fragment of the encoded protein has been shown to be capable of phosphorylating histone H2B. The particular phosphorylation catalyzed by this protein has been correlated with apoptosis, and it's possible that this protein induces the chromatin condensation observed in this process. [provided by RefSeq, Jul 2008]

Uniprot Description

MST1: a protein kinase of the STE20 family. Proteolytically activated by caspase during apoptosis. Activated by apoptotic signals as well as other stress conditions. Full activation requires both phosphorylation and caspase-mediated cleavage. Phosphorylation at serine 327 of Mst1, which is close to the caspase-3 recognition site, inhibits caspase-mediated cleavage.

Protein type: Protein kinase, STE; Autophagy; Kinase, protein; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); STE group; STE20 family; MST subfamily

Chromosomal Location of Human Ortholog: 20q11.2-q13.2

Cellular Component: nucleoplasm; cytoplasm; nucleus; cytosol

Molecular Function: protein dimerization activity; identical protein binding; protein serine/threonine kinase activity; protein serine/threonine kinase activator activity; protein binding; protein homodimerization activity; magnesium ion binding; transcription factor binding; ATP binding; receptor signaling protein serine/threonine kinase activity

Biological Process: central nervous system development; positive regulation of protein binding; apoptosis; positive regulation of apoptosis; protein amino acid autophosphorylation; cell morphogenesis; negative regulation of organ growth; signal transduction; protein amino acid phosphorylation; patterning of blood vessels; keratinocyte differentiation; negative regulation of cell proliferation; peptidyl-serine phosphorylation; positive regulation of fat cell differentiation; neural tube formation

Disease: T-cell Immunodeficiency, Recurrent Infections, And Autoimmunity With Or Without Cardiac Malformations