Western Blot (WB)
(Western Blot analysis in NCI-H460 cell line lysates (35ug/lane) using MBS6005352. This demonstrates that MBS6005352 detected the MST1 protein (arrow).)
Rabbit anti-Human MST1 Polyclonal Antibody | anti-STK4 antibody
MST1, CT (MST1, D3F15S2, DNF15S2, HGFL, Hepatocyte growth factor-like protein, Macrophage stimulatory protein, Macrophage-stimulating protein, Hepatocyte growth factor-like protein alpha chain, Hepatocyte growth factor-like protein beta chain)
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:10-50
Flow Cytometry: 1:10-50
Western Blot (WB)
(Western Blot analysis in NCI-H460 cell line lysates (35ug/lane) using MBS6005352. This demonstrates that MBS6005352 detected the MST1 protein (arrow).)
Western Blot (WB)
(Western Blot analysis in NCI-H460 cell line lysates (35ug/lane) using MBS6005352. This demonstrates that MBS6005352 detected the MST1 protein (arrow).)
Western Blot (WB)
(Western Blot analysis in 293T/17 whole cell lysate (20ug/lane) using MBS6005352 (1:1000). Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10,000 dilution. Predicted band size: 80kD. Blocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(Western Blot analysis in 293T/17 whole cell lysate (20ug/lane) using MBS6005352 (1:1000). Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10,000 dilution. Predicted band size: 80kD. Blocking/Dilution buffer: 5% NFDM/TBST.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis in formalin fixed and paraffin embedded human cerebellum tissue using MBS6005352 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS6005352 for immunohistochemistry.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis in formalin fixed and paraffin embedded human cerebellum tissue using MBS6005352 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS6005352 for immunohistochemistry.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a cytoplasmic kinase that is structurally similar to the yeast Ste20p kinase, which acts upstream of the stress-induced mitogen-activated protein kinase cascade. The encoded protein can phosphorylate myelin basic protein and undergoes autophosphorylation. A caspase-cleaved fragment of the encoded protein has been shown to be capable of phosphorylating histone H2B. The particular phosphorylation catalyzed by this protein has been correlated with apoptosis, and it's possible that this protein induces the chromatin condensation observed in this process. [provided by RefSeq, Jul 2008]
Uniprot Description
MST1: a protein kinase of the STE20 family. Proteolytically activated by caspase during apoptosis. Activated by apoptotic signals as well as other stress conditions. Full activation requires both phosphorylation and caspase-mediated cleavage. Phosphorylation at serine 327 of Mst1, which is close to the caspase-3 recognition site, inhibits caspase-mediated cleavage.
Protein type: EC 2.7.11.1; Kinase, protein; Protein kinase, STE; Protein kinase, Ser/Thr (non-receptor); Autophagy; STE group; STE20 family; MST subfamily
Chromosomal Location of Human Ortholog: 20q11.2-q13.2
Cellular Component: nucleoplasm; cytoplasm; cytosol; nucleus
Molecular Function: protein dimerization activity; protein serine/threonine kinase activator activity; identical protein binding; protein serine/threonine kinase activity; protein binding; protein homodimerization activity; magnesium ion binding; transcription factor binding; ATP binding; receptor signaling protein serine/threonine kinase activity
Biological Process: central nervous system development; positive regulation of protein binding; positive regulation of apoptosis; apoptosis; protein amino acid autophosphorylation; cell morphogenesis; negative regulation of organ growth; signal transduction; protein amino acid phosphorylation; patterning of blood vessels; keratinocyte differentiation; negative regulation of cell proliferation; peptidyl-serine phosphorylation; positive regulation of fat cell differentiation; neural tube formation
Disease: T-cell Immunodeficiency, Recurrent Infections, And Autoimmunity With Or Without Cardiac Malformations
Research Articles on STK4
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Product Notes
The STK4 stk4 (Catalog #AAA6005352) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MST1, CT (MST1, D3F15S2, DNF15S2, HGFL, Hepatocyte growth factor-like protein, Macrophage stimulatory protein, Macrophage-stimulating protein, Hepatocyte growth factor-like protein alpha chain, Hepatocyte growth factor-like protein beta chain) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MST1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:10-50 Flow Cytometry: 1:10-50. Researchers should empirically determine the suitability of the STK4 stk4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MST1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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