Thymidylate synthase, aa1-313 Human, His tag Recombinant Protein | Tyms recombinant protein

Thymidylate synthase, aa1-313, Recombinant Human, His tag (TS)

Cat. Num. AAA636603

• Gene Names: Tyms; Ts
• Purity: Affinity Purified; ~95% (SDS-PAGE) Purified by immunoaffinity chromatography.
• Synonyms: Thymidylate synthase; aa1-313 Human; His tag; aa1-313; Recombinant Human; His tag (TS); Tyms recombinant protein

• Host: E Coli
• Purity/Purification: Affinity Purified; ~95% (SDS-PAGE) Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in 20 mM Tris-HCl, pH8.0, 1mM DTT 10% glycerol
• Sequence: MGSSHHHHHH SSGLVPRGSH MPVAGSELPR RPLPPAAQER DAEPRPPHGE LQYLGQIQHI LRCGVRKDDR TGTGTLSVFG MQARYSLRDE FPLLTTKRVF WKGVLEELLW FIKGSTNAKE LSSKGVKIWD ANGSRDFLDS LGFSTREEGD LGPVYGFQWR HFGAEYRDME SDYSGQGVDQ; LQRVIDTIKT NPDDRRIIMC AWNPRDLPLM ALPPCHALCQ FYVVNSELSC QLYQRSGDMG LGVPFNIASY ALLTYMIAHI TGLKPGDFIH TLGDAHIYLN HIEPLKIQLQ REPRPFPKLR ILRKVEKIDD FKAEDFQIEG YNPHPTIKME MAV

• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for Tyms recombinant protein

Thymidylate synthase is an intracellular enzyme critical for de novo synthesis of DNA. This function maintains the dTMP(thymidine-5-prime monophosphate) pool critical for DNA replication and repair. In cancer, expression of this protein is often elevated and becomes further elevated as a result of treatment with the most commonly used chemotherapeutic, 5-fluorouracil (5-FU). Resistance or lack of response to 5-FU is attributed to the elevation of thymidylate synthase activity. Recombinant human Thymidylate synthase protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Recombinant human Thymidylate synthase protein, fused to His-tag at N-terminus, was expressed in E. coli

Product Categories/Family for Tyms recombinant protein

Molecular Biology; MB-Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 46358062
• NCBI GeneID: 22171
• NCBI Accession #: NP_067263.1
• NCBI GenBank Nucleotide #: NM_021288.4
• UniProt Accession #: P07607
• Molecular Weight: 37.8 kDa
• NCBI Official Full Name: thymidylate synthase
• NCBI Official Synonym Full Names: thymidylate synthase
• NCBI Official Symbol: Tyms
• NCBI Official Synonym Symbols: Ts
• NCBI Protein Information: thymidylate synthase; Tsase
• UniProt Protein Name: Thymidylate synthase
• UniProt Gene Name: Tyms
• UniProt Synonym Gene Names: TS; TSase
• UniProt Entry Name: TYSY_MOUSE

NCBI Description

This gene encodes an enzyme that catalyzes the methylation of deoxyuridylate to deoxythymidylate using 5,10-methylenetetrahydrofolate as a cofactor. This function maintains the thymidine-5-prime monophosphate concentration critical for DNA replication and repair. The encoded enzyme is a target for cancer chemotherapeutic agents. The majority of transcripts for this gene lack a 3' UTR (PMID: 3022294, 3444407). The stop codon in these transcripts is UAA, compared to the UAG found in the genome and longer transcripts, as the polyA site is located within the stop codon (PMID: 3444407, 2157203). A related pseudogene has been identified on chromosome 10. [provided by RefSeq, Mar 2010]

Uniprot Description

TYMS: an enzyme in the thymidylate synthase family. Polymorphisms serve as prognostic markers for determining modality of treatment in certain cancers. Genotyping helps predict toxicity to 5-FU-based chemotherapy in patients with colorectal cancer and the response to platinum-based chemotherapy in advanced NSCLC. A common polymorphism is a determinant of red blood cell folate and homocysteine concentrations. Homodimer.

Protein type: Mitochondrial; DNA repair, damage; Nucleotide Metabolism - pyrimidine; Cofactor and Vitamin Metabolism - one carbon pool by folate; EC 2.1.1.45; Methyltransferase

Cellular Component: mitochondrion; membrane; mitochondrial matrix; cytoplasm; mitochondrial inner membrane; nucleolus; nucleus

Molecular Function: mRNA binding; transferase activity; methyltransferase activity; protein homodimerization activity; nucleotide binding; drug binding; cofactor binding; thymidylate synthase activity; folic acid binding

Biological Process: methylation; nucleotide biosynthetic process; dUMP metabolic process; dTMP biosynthetic process