NTHL1, aa1-312, His-tag Recombinant Protein | NTHL1 recombinant protein

NTHL1, Recombinant, Human, aa1-312, His-tag (Endonuclease III-like protein 1, NTH1, OCTS3, EC 4.2.99.18)

Cat. Num. AAA637304

• Gene Names: NTHL1; NTH1; OCTS3
• Purity: Purified; 80% (SDS-PAGE)
• Synonyms: NTHL1; aa1-312; His-tag; Recombinant; Human; His-tag (Endonuclease III-like protein 1; NTH1; OCTS3; EC 4.2.99.18); NTHL1 recombinant protein

• Host: E Coli
• Purity/Purification: Purified; 80% (SDS-PAGE)
• Form/Format: Supplied as a liquid in 20mM Tris-HCl buffer, pH8.0, containing 0.15M sodium chloride, 1mM DTT and 40% glycerol
• Sequence: MGSSHHHHHH SSGLVPRGSH MCSPQESGMT ALSARMLTRS RSLGPGAGPR GCREEPGPLR RREAAAEARK SHSPVKRPRK AQRLRVAYEG SDSEKGEGAE PLKVPVWEPQ DWQQQLVNIR AMRNKKDAPV DHLGTEHCYD SSAPPKVRRY QVLLSLMLSS QTKDQVTAGA MQRLRARGLT VDSILQTDDA TLGKLIYPVG FWRSKVKYIK QTSAILQQHY GGDIPASVAE LVALPGVGPK MAHLAMAVAW GTVSGIAVDT HVHRIANRLR WTKKATKSPE ETRAALEEWL PRELWHEING LLVGFGQQTC LPVHPRCHAC LNQALCPAAQ GL

• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C or lower. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

SDS-Page

(SDS-PAGE analysis of N2291-43)

Related Product Information for NTHL1 recombinant protein

NTHL1 has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site. Required for the repair of both oxidative DNA damage and spontaneous mutagenic lesions

Recombinant human NTHL1 protein fused to His-tag at the N-terminus; expressed in E. coli

Product Categories/Family for NTHL1 recombinant protein

Molecular Biology; MB-Enzymes; Endonuclease

NCBI and Uniprot Product Information

• NCBI GI #: 38197140
• NCBI GeneID: 4913
• UniProt Accession #: P78549; Q1MVR1; Q99566; Q99794; Q9BPX2
• Molecular Weight: 36.6kD
• NCBI Official Full Name: NTHL1 protein, partial
• NCBI Official Synonym Full Names: nth endonuclease III-like 1 (E. coli)
• NCBI Official Symbol: NTHL1
• NCBI Official Synonym Symbols: NTH1; OCTS3
• NCBI Protein Information: endonuclease III-like protein 1
• UniProt Protein Name: Endonuclease III-like protein 1
• Protein Family: Endonuclease III-like protein
• UniProt Gene Name: NTHL1
• UniProt Synonym Gene Names: NTH1; OCTS3
• UniProt Entry Name: NTHL1_HUMAN

NCBI Description

The protein encoded by this gene is a DNA N-glycosylase of the endonuclease III family. Like a similar protein in E. coli, the encoded protein has DNA glycosylase activity on DNA substrates containing oxidized pyrimidine residues and has apurinic/apyrimidinic lyase activity. [provided by RefSeq, Oct 2008]

Uniprot Description

NTHL1: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site. Required for the repair of both oxidative DNA damage and spontaneous mutagenic lesions. Belongs to the Nth/MutY family.

Protein type: Lyase; Hydrolase; DNA repair, damage; Mitochondrial; EC 4.2.99.18

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: nucleoplasm; mitochondrion; nucleus

Molecular Function: protein binding; DNA-(apurinic or apyrimidinic site) lyase activity; metal ion binding; endonuclease activity; 4 iron, 4 sulfur cluster binding; double-stranded DNA binding; DNA N-glycosylase activity

Biological Process: base-excision repair, AP site formation; depyrimidination; base-excision repair; DNA repair; DNA catabolic process, endonucleolytic; nucleotide-excision repair, DNA incision, 5'-to lesion

Disease: Familial Adenomatous Polyposis 3