TREML2 active protein
TREML2, Recombinant, Human (Triggering Receptor Expressed on Monocytes Cells-like Protein 2, TREM-like Transcript 2 Protein, TLT-2, C6orf76, TLT2, UNQ6268/PRO20473)
Gene Names
TREML2; TLT2; TLT-2; C6orf76; dJ238O23.1
Purity
Highly Purified~90% (SDS-PAGE, reducing conditions)
Synonyms
TREML2; Recombinant; Human (Triggering Receptor Expressed on Monocytes Cells-like Protein 2; TREM-like Transcript 2 Protein; TLT-2; C6orf76; TLT2; UNQ6268/PRO20473); TREML2 active protein
Purity/Purification
Highly Purified
~90% (SDS-PAGE, reducing conditions)
~90% (SDS-PAGE, reducing conditions)
Form/Format
Supplied as a lyophilized powder from PBS. Reconstitute with sterile PBS.
Application Notes
SDS-PAGE: 75-100kD, reducing conditions
Activity
Measured by its ability to inhibit antiCD3induced proliferation of stimulated human T cells. Human PBMC cultured for 72 hours with PHA were incubated for an additional 3 days in 96 well plates coated with antiCD3 and recombinant human TREML2/Fc Chimera. The ED50 for this effect is typically 0.45-1.8ug/ml.
Endotoxin Level
1EU/1ug (LAL)
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for TREML2 active protein
Tremlike transcript 2 protein (TLT2; also TREML2) is a 33kD (unglycosylated) type I transmembrane cell surface receptor and member of the Trem family of receptor proteins. Human TLT2 is synthesized as a 321aa precursor that contains an 18 aa signal sequence, a 250aa extracellular domain (ECD), a 21aa transmembrane segment, and a 32aa cytoplasmic tail (SwissProt # Q5T2D2). Within the ECD are an Iglike Vtype domain (aa20121) and one potential site of Nlinked glycosylation. Mature human TLT2 shares 49% aa sequence identity with mature mouse TLT2. Cell surface expression of TLT2 is seen for most of the B and myeloid cell lines. TLT2 is also expressed constitutively on CD8+ T cells and induced on CD4+ T cells after activation. TLT2 plays a role in the innate and adaptive immune responses. In 2008, Hashiguchi et al. discovered that the B7 family member B7H3, which also plays important roles in immune responses, specifically bound to TLT2. According to their research, stimulation with B7H3 transfectants preferentially upregulated the proliferation and IFN-gamma production on CD8+ T cells. Also, transduction of TLT2 into T cells resulted in enhanced IL2 and IFN-gamma production via interactions with B7H3, and blockade of the B7H3:TLT2 pathway with mAb against B7H3 or TLT2 efficiently inhibited contact hypersensitivity responses. In 2009, however, Leitner et al. extensively analyzed interaction of B7H3 with TLT2 and found no evidence for such an interaction.
Product Categories/Family for TREML2 active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
~54kD
NCBI Official Full Name
trem-like transcript 2 protein
NCBI Official Synonym Full Names
triggering receptor expressed on myeloid cells-like 2
NCBI Official Symbol
TREML2
NCBI Official Synonym Symbols
TLT2; TLT-2; C6orf76; dJ238O23.1
NCBI Protein Information
trem-like transcript 2 protein; triggering receptor expressed on myeloid cells-like protein 2
UniProt Protein Name
Trem-like transcript 2 protein
Protein Family
UniProt Gene Name
TREML2
UniProt Synonym Gene Names
C6orf76; TLT2; TLT-2
UniProt Entry Name
TRML2_HUMAN
NCBI Description
TREML2 is located in a gene cluster on chromosome 6 with the single Ig variable (IgV) domain activating receptors TREM1 (MIM 605085) and TREM2 (MIM 605086), but it has distinct structural and functional properties (Allcock et al., 2003 [PubMed 12645956]).[supplied by OMIM, Mar 2008]
Uniprot Description
TREML2: Cell surface receptor that may play a role in the innate and adaptive immune response. Acts as a counter-receptor for CD276 and interaction with CD276 on T-cells enhances T-cell activation.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 6p21.1
Cellular Component: cell surface; plasma membrane; integral to membrane
Molecular Function: protein binding; receptor activity
Biological Process: T cell activation
Research Articles on TREML2
Similar Products
Product Notes
The TREML2 treml2 (Catalog #AAA635979) is an Active Protein and is intended for research purposes only. The product is available for immediate purchase. SDS-PAGE: 75-100kD, reducing conditions. Researchers should empirically determine the suitability of the TREML2 treml2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TREML2, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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