Neurotrophic Tyrosine Kinase Receptor Type 2 Recombinant Protein | NTRK2 recombinant protein

Recombinant Neurotrophic Tyrosine Kinase Receptor Type 2 (NTRK2)

Cat. Num. AAA2028837

• Gene Names: NTRK2; TRKB; trk-B; GP145-TrkB
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Neurotrophic Tyrosine Kinase Receptor Type 2; Recombinant Neurotrophic Tyrosine Kinase Receptor Type 2 (NTRK2); NTRK2 recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; CPTSCKCSA SRIWCSDPSP GIVAFPRLEP NSVDPENITE IFIANQKRLE IINEDDVEAY VGLRNLTIVD SGLKFVAHKA FLKNSNLQHI NFTRNKLTSL SRKHFRHLDL SELILVGNPF TCSCDIMWIK TLQEAKSSPD TQDLYCLNES SKNIPLANLQ IPNCGLPSAN LAAPNLTVEE GKSITLSCSV AGDPVPNMYW DVGNLVSKHM NETSHTQGSL RITNISSDDS GKQISCVAEN LVGEDQDSVN LTVHFAPTIT FLESPTSDHH WCIPFTVKGN PKPALQWFYN GAILNESKYI CTKIHVTNHT EYHGCLQLDN PTHMNNGDYT LIAKNEYGKD EKQISAHFMG WPGIDDGANPNYPDVIYEDY GTAANDIGDT TNRSNEIPST DVTDKTGREH
• Sequence Length: 822

• Applicable Applications for NTRK2 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 47.9KDa
• Accurate Molecular Mass (KD): 48kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: N-terminal His-Tag
• Organism Species: Homo sapiens (Human)
• Fragment: Cys32~His430
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 15217077
• NCBI GeneID: 4915
• Molecular Weight: 35,332 Da
• NCBI Official Full Name: neurotrophic tyrosine kinase receptor type 2
• NCBI Official Synonym Full Names: neurotrophic tyrosine kinase, receptor, type 2
• NCBI Official Symbol: NTRK2
• NCBI Official Synonym Symbols: TRKB; trk-B; GP145-TrkB
• NCBI Protein Information: BDNF/NT-3 growth factors receptor
• UniProt Protein Name: BDNF/NT-3 growth factors receptor
• Protein Family: BDNF/NT-3 growth factors receptor
• UniProt Gene Name: NTRK2
• UniProt Synonym Gene Names: TRKB; Trk-B
• UniProt Entry Name: NTRK2_HUMAN

NCBI Description

This gene encodes a member of the neurotrophic tyrosine receptor kinase (NTRK) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. Signalling through this kinase leads to cell differentiation. Mutations in this gene have been associated with obesity and mood disorders. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]

Uniprot Description

TrkB: a receptor tyrosine kinase of the Trk family. Receptor for brain-derived neurotrophic factor (BDNF), neurotrophin-3, -4 and -5 but not nerve growth factor (NGF). Involved in the development and maintenance of the nervous system. Three splice variant isoforms have been described.

Protein type: Protein kinase, TK; Kinase, protein; EC 2.7.10.1; Membrane protein, integral; Protein kinase, tyrosine (receptor); TK group; Trk family

Chromosomal Location of Human Ortholog: 9q22.1

Cellular Component: postsynaptic membrane; cell surface; growth cone; rough endoplasmic reticulum; integral to plasma membrane; presynaptic active zone; dendritic spine; terminal button; excitatory synapse; perikaryon; endosome membrane; cytosol; receptor complex

Molecular Function: brain-derived neurotrophic factor receptor activity; neurotrophin binding; protein homodimerization activity; ephrin receptor binding; brain-derived neurotrophic factor binding; ATP binding

Biological Process: circadian rhythm; mechanoreceptor differentiation; retinal rod cell development; peptidyl-tyrosine phosphorylation; nerve growth factor receptor signaling pathway; protein amino acid autophosphorylation; neuron migration; regulation of dendrite development; regulation of neurotransmitter secretion; positive regulation of synaptic transmission, glutamatergic; neuron differentiation; positive regulation of MAPKKK cascade; positive regulation of cell proliferation; oligodendrocyte differentiation; feeding behavior; negative regulation of neuron apoptosis; inflammatory response; vasculogenesis; neuromuscular junction development; aging; central nervous system neuron development; response to light stimulus; brain-derived neurotrophic factor receptor signaling pathway; adenylate cyclase activation; regulation of protein kinase B signaling cascade; learning; positive regulation of peptidyl-serine phosphorylation; peripheral nervous system neuron development; positive regulation of phosphoinositide 3-kinase cascade; long-term memory; glutamate secretion; positive regulation of axonogenesis; cerebral cortex development; transmembrane receptor protein tyrosine kinase signaling pathway

Disease: Obesity, Hyperphagia, And Developmental Delay