TREML2 recombinant protein

TREML2 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8120899

• Gene Names: TREML2; TLT2; TLT-2; C6orf76; FLJ13693; MGC149715; MGC149716; dJ238O23.1
• Purity: >95% as determined by SDS-PAGE
• Synonyms: TREML2; TREML2 Protein; Human; Recombinant (His Tag); Human TREML2/TLT2 Protein (His Tag); C6orf76 Protein; dJ238O23.1 Protein; FLJ13693 Protein; MGC149715 Protein; MGC149716 Protein; TLT-2 Protein; TLT2 Protein; UNQ6268/PRO20473 Protein; triggering receptor expressed on myeloid cells like 2; TREML2 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Ser268

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Gly 19
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human TREML2 (NP_079083.2) extracellular domain (Met 1-Ser 268) was fused with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for TREML2 recombinant protein

Background: Trem-like transcript 2 protein, also known as Triggering receptor expressed on myeloid cells-like protein 2, TREML2 and TLT2, is a single-pass type I membrane protein that contains one Ig-like V-type (immunoglobulin-like) domain. TREML2 is detected in cultured B cells, T cell leukemia and monocyte leukemia. TREML2 is expressed constitutively on CD8 T-cells and induced on CD4 T-cells after activation. TREML2 is a cell surface receptor that may play a role in the innate and adaptive immune response. TREML2 acts as a counter-receptor for CD276 and interaction with CD276 on T-cells enhances T-cell activation. Murine B7-H3 is specifically bound to Triggering receptor expressed on myeloid cells (TREM)-like transcript 2 (TLT-2, TREML2). TREML2 was expressed on CD8(+) T cells constitutively and on activated CD4(+) T cells. Stimulation with B7-H3 transfectants preferentially up-regulated the proliferation and IFN-gamma production of CD8(+) T cells. Transduction of TREML2 into T cells resulted in enhanced IL-2 and IFN-gamma production via interactions with B7-H3. There may be a direct interaction between B7-H3 and TREML2 that preferentially enhances CD8(+) T cell activation.

Product Categories/Family for TREML2 recombinant protein

ITIM/ITAM Immunoreceptors and Related Molecules

References

Mungall A.J. et al., 2003, Nature. 425: 805-11. Clark HF. et al., 2003, Genome Res. 13: 2265-70. The MGC Project Team. et al., 2004, Genome Res. 14:2121-7. Hashiguchi M., et al., 2008, Proc. Natl.Acad. Sci. USA.105:10495-500. Leitner,J. et al., 2009, Eur J Immunol. 39 (7):1754-64.

NCBI and Uniprot Product Information

• NCBI GI #: 170932501
• NCBI GeneID: 79865
• NCBI Accession #: NP_079083.2
• NCBI GenBank Nucleotide #: NM_024807.2
• UniProt Accession #: Q5T2D2; Q08AP8; Q08AP9; Q8IWY0; Q9H8E9
• Molecular Weight: 35,127 Da
• NCBI Official Full Name: trem-like transcript 2 protein
• NCBI Official Synonym Full Names: triggering receptor expressed on myeloid cells-like 2
• NCBI Official Symbol: TREML2
• NCBI Official Synonym Symbols: TLT2; TLT-2; C6orf76; FLJ13693; MGC149715; MGC149716; dJ238O23.1
• NCBI Protein Information: trem-like transcript 2 protein; OTTHUMP00000017974; triggering receptor expressed on myeloid cells-like protein 2
• UniProt Protein Name: Trem-like transcript 2 protein
• Protein Family: Trem-like transcript 2 protein
• UniProt Gene Name: TREML2
• UniProt Synonym Gene Names: C6orf76; TLT2
• UniProt Entry Name: TRML2_HUMAN

NCBI Description

TREML2 is located in a gene cluster on chromosome 6 with the single Ig variable (IgV) domain activating receptors TREM1 (MIM 605085) and TREM2 (MIM 605086), but it has distinct structural and functional properties (Allcock et al., 2003 [PubMed 12645956]).[supplied by OMIM]

Uniprot Description

TREML2: Cell surface receptor that may play a role in the innate and adaptive immune response. Acts as a counter-receptor for CD276 and interaction with CD276 on T-cells enhances T-cell activation.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 6p21.1

Cellular Component: cell surface; integral to membrane; plasma membrane

Molecular Function: protein binding; receptor activity

Biological Process: T cell activation