Akt1, active, Western Blot Positive Control Recombinant Protein
Akt1, Human, phosphorylated, active, Western Blot Positive Control (v-akt Mouse Thymoma Viral Oncogene Homolog 1, C-AKT, MGC99656, PKB, PRKBA, Protein kinase B, RAC, RAC-ALPHA, RAC-alpha serine/threonine-protein kinase, RAC-PK-alpha) Recombinant
Applications
Western Blot
Purity
Highly PurifiedHighly purified (90%).
Synonyms
Akt1; active; Western Blot Positive Control; Human; phosphorylated; Western Blot Positive Control (v-akt Mouse Thymoma Viral Oncogene Homolog 1; C-AKT; MGC99656; PKB; PRKBA; Protein kinase B; RAC; RAC-ALPHA; RAC-alpha serine/threonine-protein kinase; RAC-PK-alpha) Recombinant; Western Blot Positive Control recombinant protein
Specificity
This preparation was active (Specific Activity: 300-1800u/mg). AKT1 was activated with MAPKAP kinase 2 and PDK1 and repurified.
Purity/Purification
Highly Purified
Highly purified (90%).
Highly purified (90%).
Form/Format
Supplied in SDS-PAGE sample buffer (reduced). This preparation of AKT1 is phosphorylated but not active in sample buffer.
Applicable Applications for Akt1, active, Western Blot Positive Control recombinant protein
Western Blot (WB)
Application Notes
Suitable for use in Western Blot.
Dilution: Western Blot: Load ~10 ul/lane.
Optimal dilutions to be determined by researcher.
Dilution: Western Blot: Load ~10 ul/lane.
Optimal dilutions to be determined by researcher.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 6-12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for Akt1, active, Western Blot Positive Control recombinant protein
Putative human homologs of the protooncogene v-akt of the acutely transforming retrovirus AKT8 have been cloned. These protein-serine/threonine kinase proteins have a catalytic domain closely related to both PKA and PKC and have been designated rac (related to A and C kinases), pkb (Protein kinase B) or Akt. RAC protein kinase family members feature pleckstrin homology (PH) domain at the amino terminus and a protein-serine/threonine kinase catalytic domain at the C-terminus. The N-terminal domain (referred to as AH-Akt Homology domain) spans from 1-148 amino acids and contains the PH domain, a region found in diverse group of signaling proteins. The PH domain (aa1-106) has been implicated in interactions with other proteins such as G-protein bg subunits, as well as phosphoinositides. The kinase domain is located between residues 148-411. These enzymes are activated by diverse ligands such as PGDF, EGF and basic FG in NIH 3T3, Rat-1 or Swiss-3T3 cells.
Product Categories/Family for Akt1, active, Western Blot Positive Control recombinant protein
Similar Products
Product Notes
The Akt1, active, Western Blot Positive Control (Catalog #AAA635664) is a Recombinant Protein and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Akt1, active, Western Blot Positive Control can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Dilution: Western Blot: Load ~10 ul/lane. Optimal dilutions to be determined by researcher. Researchers should empirically determine the suitability of the Akt1, active, Western Blot Positive Control for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Akt1, active, Western Blot Positive Control, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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