Rabbit anti-Human TBCE Polyclonal Antibody | anti-TBCE antibody

TBCE, ID (TBCE, Tubulin-specific chaperone E, Tubulin-folding cofactor E) (PE)

Cat. Num. AAA6354050

• Gene Names: TBCE; HRD; KCS; KCS1; pac2; PEAMO
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: TBCE; Polyclonal Antibody; ID (TBCE; Tubulin-specific chaperone E; Tubulin-folding cofactor E) (PE); Tubulin-folding cofactor E; anti-TBCE antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-TBCE antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: TBCE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 371~400 amino acids from the Central region of human TBCE.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(TBCE Antibody (Center) western blot analysis in CEM,K562 cell line lysates (35ug/lane).This demonstrates the TBCE antibody detected the TBCE protein (arrow).)

Related Product Information for anti-TBCE antibody

Cofactor E is one of four proteins (cofactors A, D, E, and C) involved in the pathway leading to correctly folded beta-tubulin from folding intermediates. Cofactors A and D are believed to play a role in capturing and stabilizing beta-tubulin intermediates in a quasi-native confirmation. Cofactor E binds to the cofactor D/beta-tubulin complex; interaction with cofactor C then causes the release of beta-tubulin polypeptides that are committed to the native state.

Product Categories/Family for anti-TBCE antibody

Antibodies; Tubulin

References

Biernacki, M.A., et al. Cancer Res. 70(3):906-915(2010). Padidela, R., et al. J. Clin. Endocrinol. Metab. 94(8):2686-2691(2009). Lindgren, C.M., et al. PLoS Genet. 5 (6), E1000508 (2009). Jin, S., et al. Development 136(9):1571-1581(2009). Diaz, G.A., et al. Genomics 54(1):13-18(1998). Parvari, R., et al. Am. J. Hum. Genet. 63(1):163-169(1998).

NCBI and Uniprot Product Information

• NCBI GI #: 4507375
• NCBI GeneID: 6905
• NCBI Accession #: NP_003184
• NCBI GenBank Nucleotide #: NM_003193.5
• UniProt Accession #: Q15813
• Molecular Weight: 57kDa
• NCBI Official Full Name: tubulin-specific chaperone E isoform a
• NCBI Official Synonym Full Names: tubulin folding cofactor E
• NCBI Official Symbol: TBCE
• NCBI Official Synonym Symbols: HRD; KCS; KCS1; pac2; PEAMO
• NCBI Protein Information: tubulin-specific chaperone E
• UniProt Protein Name: Tubulin-specific chaperone E
• Protein Family: Tubulin-specific chaperone
• UniProt Gene Name: TBCE
• UniProt Entry Name: TBCE_HUMAN

NCBI Description

Cofactor E is one of four proteins (cofactors A, D, E, and C) involved in the pathway leading to correctly folded beta-tubulin from folding intermediates. Cofactors A and D are believed to play a role in capturing and stabilizing beta-tubulin intermediates in a quasi-native confirmation. Cofactor E binds to the cofactor D/beta-tubulin complex; interaction with cofactor C then causes the release of beta-tubulin polypeptides that are committed to the native state. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Tubulin-folding protein; involved in the second step of the tubulin folding pathway. Seems to be implicated in the maintenance of the neuronal microtubule network. Involved in regulation of tubulin heterodimer dissociation. Ref.5

Subunit structure: Supercomplex made of cofactors A to E. Cofactors A and D function by capturing and stabilizing tubulin in a quasi-native conformation. Cofactor E binds to the cofactor D-tubulin complex; interaction with cofactor C then causes the release of tubulin polypeptides that are committed to the native state. Cofactors B and E can form a heterodimer which binds to alpha-tubulin and enhances their ability to dissociate tubulin heterodimers.

Subcellular location: Cytoplasm

By similarity. Cytoplasm › cytoskeleton

By similarity.

Involvement in disease: Hypoparathyroidism-retardation-dysmorphism syndrome (HRD) [MIM:241410]: Autosomal recessive disorder reported almost exclusively in Middle Eastern populations.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.10Kenny-Caffey syndrome 1 (KCS1) [MIM:244460]: An autosomal recessive form of Kenny-Caffey syndrome, a disorder characterized by impaired skeletal development with small and dense bones, short stature, and primary hypoparathyroidism with hypocalcemia. Clinical features include cortical thickening and medullary stenosis of the tubular bones, delayed closure of fontanels, defective dentition, small eyes with hypermetropia, and frontal bossing with a triangular face.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.10

Sequence similarities: Belongs to the TBCE family.Contains 1 CAP-Gly domain.Contains 7 LRR (leucine-rich) repeats.Contains 1 LRRCT domain.