Rabbit anti-Human SSX3 Polyclonal Antibody | anti-SSX3 antibody

SSX3, ID (SSX3, Protein SSX3, Cancer/testis antigen 5.3) (Azide free) (HRP)

Cat. Num. AAA6351426

• Gene Names: SSX3; CT5.3
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SSX3; Polyclonal Antibody; ID (SSX3; Protein SSX3; Cancer/testis antigen 5.3) (Azide free) (HRP); Cancer/testis antigen 5.3; anti-SSX3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-SSX3 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: SSX3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 52-80 amino acids from the Central region of human SSX3.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(SSX3 Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the SSX3 Antibody detected the SSX3 protein (arrow).)

Related Product Information for anti-SSX3 antibody

The product of this gene belongs to the family of highly homologous synovial sarcoma X (SSX) breakpoint proteins. These proteins may function as transcriptional repressors. They are also capable of eliciting spontaneously humoral and cellular immune responses in cancer patients, and are potentially useful targets in cancer vaccine-based immunotherapy. SSX1, SSX2 and SSX4 genes have been involved in the t(X;18) translocation characteristically found in all synovial sarcomas. This gene appears not to be involved in this type of chromosome translocation. Two transcript variants encoding distinct isoforms have been identified for this gene.

Product Categories/Family for anti-SSX3 antibody

Antibodies; Cancer Markers

References

Ross, M.T., et al. Nature 434(7031):325-337(2005). Gure, A.O., et al. Int. J. Cancer 101(5):448-453(2002). de Bruijn, D.R., et al. Genes Chromosomes Cancer 34(3):285-298(2002). Gure, A.O., et al. Int. J. Cancer 72(6):965-971(1997). de Leeuw, B., et al. Cytogenet. Cell Genet. 73(3):179-183(1996).

NCBI and Uniprot Product Information

• NCBI GI #: 10337601
• NCBI GeneID: 10214
• NCBI Accession #: NP_066294.1
• NCBI GenBank Nucleotide #: NM_021014.3
• UniProt Accession #: Q99909; O60223; Q5JQZ3; Q9BRW7
• Molecular Weight: 19,457 Da
• NCBI Official Full Name: protein SSX3
• NCBI Official Synonym Full Names: SSX family member 3
• NCBI Official Symbol: SSX3
• NCBI Official Synonym Symbols: CT5.3
• NCBI Protein Information: protein SSX3
• UniProt Protein Name: Protein SSX3
• Protein Family: Protein
• UniProt Gene Name: SSX3
• UniProt Synonym Gene Names: CT5.3

NCBI Description

The product of this gene belongs to the family of highly homologous synovial sarcoma X (SSX) breakpoint proteins. These proteins may function as transcriptional repressors. They are also capable of eliciting spontaneous humoral and cellular immune responses in cancer patients, and are potentially useful targets in cancer vaccine-based immunotherapy. While some of the related SSX genes are involved in t(X;18)(p11.2;q11.2) translocations that are characteristically found in all synovial sarcomas, this gene does not appear to be involved in such translocations. [provided by RefSeq, Jul 2013]

Uniprot Description

SSX3: Could act as a modulator of transcription. Belongs to the SSX family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cancer Testis Antigen (CTA)

Chromosomal Location of Human Ortholog: Xp11.23

Cellular Component: nucleus

Molecular Function: nucleic acid binding; protein binding; transcription corepressor activity

Biological Process: regulation of transcription, DNA-templated; transcription, DNA-dependent