Rabbit anti-Human, Mouse SCN1B Polyclonal Antibody | anti-SCN1B antibody

SCN1B, NT (SCN1B, Sodium channel subunit beta-1) (AP)

Cat. Num. AAA6345349

• Gene Names: SCN1B; ATFB13; BRGDA5; GEFSP1
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: SCN1B; Polyclonal Antibody; NT (SCN1B; Sodium channel subunit beta-1) (AP); Sodium channel subunit beta-1; anti-SCN1B antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human SCN1B. Species crossreactivity: mouse
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-SCN1B antibody: Immunohistochemistry (IHC) Paraffin, ELISA (EIA), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 63-90 in the N-terminal region of human SCN1B. Species sequence homology: bovine, rabbit, rat
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in mouse Neuro-2a cell line lysates (35ug/lane) usingMBS6003566. This demonstrates that MBS6003566 detected the SCN1B protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue using MBS6003566 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS6003566 for immunohistochemistry.)

Testing Data

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Product Categories/Family for anti-SCN1B antibody

Antibodies; Ion Channel

References

1. Grover, S., et al. Pharmacogenomics 11(7):927-941(2010). 2. Tan, B.H., et al. Heart Rhythm 7(6):771-778(2010). 3. Casini, S., et al. Cardiovasc. Res. 85(4):691-700(2010). 4. Ogawa, R., et al. Int J Clin Pharmacol Ther 48(2):109-119(2010). 5. Watanabe, H., et al. Circ Arrhythm Electrophysiol 2(3):268-275(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 39930610
• NCBI GeneID: 6324
• NCBI Accession #: NP_950238.1
• NCBI GenBank Nucleotide #: NM_199037.3
• UniProt Accession #: Q07699; Q5TZZ4; Q6TN97
• Molecular Weight: 30,440 Da
• NCBI Official Full Name: sodium channel subunit beta-1 isoform b
• NCBI Official Synonym Full Names: sodium channel, voltage gated, type I beta subunit
• NCBI Official Symbol: SCN1B
• NCBI Official Synonym Symbols: ATFB13; BRGDA5; GEFSP1
• NCBI Protein Information: sodium channel subunit beta-1; sodium channel beta-1 subunit; sodium channel, voltage-gated, type I, beta subunit
• UniProt Protein Name: Sodium channel subunit beta-1
• Protein Family: Sodium channel
• UniProt Gene Name: SCN1B
• UniProt Entry Name: SCN1B_HUMAN

NCBI Description

Voltage-gated sodium channels are heteromeric proteins that function in the generation and propagation of action potentials in muscle and neuronal cells. They are composed of one alpha and two beta subunits, where the alpha subunit provides channel activity and the beta-1 subunit modulates the kinetics of channel inactivation. This gene encodes a sodium channel beta-1 subunit. Mutations in this gene result in generalized epilepsy with febrile seizures plus, Brugada syndrome 5, and defects in cardiac conduction. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Oct 2009]

Uniprot Description

SCN1B: Crucial in the assembly, expression, and functional modulation of the heterotrimeric complex of the sodium channel. The subunit beta-1 can modulate multiple alpha subunit isoforms from brain, skeletal muscle, and heart. Its association with neurofascin may target the sodium channels to the nodes of Ranvier of developing axons and retain these channels at the nodes in mature myelinated axons. Defects in SCN1B are the cause of generalized epilepsy with febrile seizures plus type 1 (GEFS+1). Generalized epilepsy with febrile seizures-plus refers to a rare autosomal dominant, familial condition with incomplete penetrance and large intrafamilial variability. Patients display febrile seizures persisting sometimes beyond the age of 6 years and/or a variety of afebrile seizure types. GEFS+ is a disease combining febrile seizures, generalized seizures often precipitated by fever at age 6 years or more, and partial seizures, with a variable degree of severity. Defects in SCN1B are the cause of Brugada syndrome type 5 (BRGDA5). A tachyarrhythmia characterized by right bundle branch block and ST segment elevation on an electrocardiogram (ECG). It can cause the ventricles to beat so fast that the blood is prevented from circulating efficiently in the body. When this situation occurs (called ventricular fibrillation), the individual will faint and may die in a few minutes if the heart is not reset. Belongs to the sodium channel auxiliary subunit SCN1B (TC 8.A.17) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Channel, sodium

Chromosomal Location of Human Ortholog: 19q13.1

Cellular Component: voltage-gated sodium channel complex; T-tubule; extracellular region

Molecular Function: sodium channel inhibitor activity; sodium channel regulator activity; voltage-gated sodium channel activity

Biological Process: synaptic transmission; membrane depolarization; axon guidance; action potential propagation; corticospinal neuron axon guidance; cell adhesion; locomotion; response to pyrethroid; cardiac muscle contraction

Disease: Generalized Epilepsy With Febrile Seizures Plus, Type 1; Atrial Fibrillation, Familial, 13; Brugada Syndrome 5