Rabbit anti-Human, Mouse RPL30 Polyclonal Antibody | anti-RPL30 antibody

RPL30, NT (RPL30, 60S ribosomal protein L30) (AP)

Cat. Num. AAA6343479

• Gene Names: RPL30; L30
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RPL30; Polyclonal Antibody; NT (RPL30; 60S ribosomal protein L30) (AP); 60S ribosomal protein L30; anti-RPL30 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-RPL30 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: RPL30 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human RPL30.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(RPL30 Antibody (N-term) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the RPL30 antibody detected the RPL30 protein (arrow).)

Western Blot (WB)

(RPL30 Antibody (N-term) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the RPL30 antibody detected the RPL30 protein (arrow).)

Related Product Information for anti-RPL30 antibody

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L30E family of ribosomal proteins. It is located in the cytoplasm. This gene is co-transcribed with the U72 small nucleolar RNA gene, which is located in its fourth intron. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq].

Product Categories/Family for anti-RPL30 antibody

Antibodies; Ribosomal Proteins

References

Sun, X.X., et al. J. Biol. Chem. 285(33):25812-25821(2010). Beausoleil, S.A., et al. Nat. Biotechnol. 24(10):1285-1292(2006). De Bortoli, M., et al. BMC Cancer 6, 223 (2006): Bouwmeester, T., et al. Nat. Cell Biol. 6(2):97-105(2004). Kapp, L.D., et al. Annu. Rev. Biochem. 73, 657-704 (2004).

NCBI and Uniprot Product Information

• NCBI GI #: 4506631
• NCBI GeneID: 6156
• NCBI Accession #: NP_000980.1
• NCBI GenBank Nucleotide #: NM_000989.3
• UniProt Accession #: P62888; P04645; Q502Z6; B2R591
• Molecular Weight: 12,784 Da
• NCBI Official Full Name: 60S ribosomal protein L30
• NCBI Official Synonym Full Names: ribosomal protein L30
• NCBI Official Symbol: RPL30
• NCBI Official Synonym Symbols: L30
• NCBI Protein Information: 60S ribosomal protein L30
• UniProt Protein Name: 60S ribosomal protein L30
• Protein Family: 60S ribosomal protein
• UniProt Gene Name: RPL30
• UniProt Entry Name: RL30_HUMAN

NCBI Description

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L30E family of ribosomal proteins. It is located in the cytoplasm. This gene is co-transcribed with the U72 small nucleolar RNA gene, which is located in its fourth intron. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]

Uniprot Description

RPL30: a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L30E family of ribosomal proteins. It is located in the cytoplasm. This gene is co-transcribed with the U72 small nucleolar RNA gene, which is located in its fourth intron. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]

Protein type: Ribosomal; Translation

Chromosomal Location of Human Ortholog: 8q22

Cellular Component: focal adhesion; membrane; cytoplasm; nucleus; cytosol

Molecular Function: protein binding; structural constituent of ribosome; RNA binding

Biological Process: SRP-dependent cotranslational protein targeting to membrane; cellular protein metabolic process; translational elongation; viral reproduction; translation; translational initiation; mRNA catabolic process, nonsense-mediated decay; viral transcription; gene expression; viral infectious cycle; translational termination