Rabbit anti-Mouse ATRIP (Ser239) Polyclonal Antibody | anti-ATRIP antibody

Anti-Phospho-Ser239 ATRIP

Cat. Num. AAA502187

• Reactivity: Mouse
• Applications: Western Blot
• Purity: Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.)
• Synonyms: ATRIP (Ser239); Polyclonal Antibody; Anti-Phospho-Ser239 ATRIP; anti-ATRIP antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Specificity: Specific for the ~86k ATRIP protein phosphorylated at Ser239. The antibody also recognizes a band at ~32k.
• Purity/Purification: Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.)
• Form/Format: 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
• Sequence Length: 664

• Applicable Applications for anti-ATRIP antibody: Western Blot (WB)
• Application Notes: Quality Control: Western blots performed on each lot.; WB: 1:500
• Antigen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser239 of human ATRIP.
• Immunogen Information: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser239 conjugated to KLH
• Immunogen Species: Human
• Reactivity Assumed Based on 100% Sequence Homology: Bovine, human, non-human primate and sheep
• Species Reactivity Note: The antibody has been directly tested for reactivity in Western blots with mouse tissue. It is anticipated that the antibody will also react with bovine, human, non-human primates and sheep based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
• Biological Significance: ATRIP, ATR interacting protein, binds to ATR to regulate ATR expression, and is an essential component of the DNA damage checkpoint pathway (Cortez et al, 2001). ATR is recruited to DNA lesions in part through its association with ATRIP, which in turn interacts with the single-stranded DNA binding protein RPA (Ball et al, 2007). DNA replication forks may stall as a result of DNA damage causing phosphorylation of several proteins, including BRCA1 when colocalizing with ATR/ATRIP complex and RPA (Venere et al, 2007). The DNA replication fork stall coincides with BRCA1 directly phosphorylating ATRIP at ser239 (Venere et al, 2007).
• Preparation and Storage: Store at -20 degree C; stable for at least 1 year

Testing Data

Related Product Information for anti-ATRIP antibody

Affinity purified rabbit polyclonal antibody

References

• Cortez D, Guntuku S, Qin J, Elledge SJ (2001) ATR and ATRIP: partners in checkpoint signaling. Science 294(5547):1713-6.
• Ball HL, Ehrhardt MR, Mordes DA, Glick GG, Chazin WJ, Cortex D. (2007) Function of a conserved checkpoint recruitment domain in ATRIP proteins. Mol Cel Biol. 27(9):3367-77.
• Venere M, Synder A, Zgheib, and Halazonetis T. (2007) Phosphorylation of ATR-intereacting Protien on Ser239 Mediates an Interaction with Breast-Ovarian Cancer Susceptibility 1 and Checkpoint Function. Cancer Res 67(13): 6100-5.

NCBI and Uniprot Product Information

• NCBI GI #: 402534571
• NCBI GeneID: 84126
• NCBI Accession #: NP_001257951.1
• NCBI GenBank Nucleotide #: NM_001271022.1
• UniProt Accession #: Q8WXE1; Q69YK9; Q8NHQ2; Q8WUG7; Q96CL3; Q9HA30; A8K6A3; A8K714; B2RCE7; B4DU92; B5MEB7
• Molecular Weight: 86
• NCBI Official Full Name: ATR-interacting protein isoform 3
• NCBI Official Synonym Full Names: ATR interacting protein
• NCBI Official Symbol: ATRIP
• NCBI Protein Information: ATR-interacting protein; ATM and Rad3-related-interacting protein
• UniProt Protein Name: ATR-interacting protein
• Protein Family: ATR-interacting protein
• UniProt Gene Name: ATRIP
• UniProt Synonym Gene Names: AGS1
• UniProt Entry Name: ATRIP_HUMAN

NCBI Description

This gene encodes an essential component of the DNA damage checkpoint. The encoded protein binds to single-stranded DNA coated with replication protein A. The protein also interacts with the ataxia telangiectasia and Rad3 related protein kinase, resulting in its accumulation at intranuclear foci induced by DNA damage. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2012]

Uniprot Description

Function: Required for checkpoint signaling after DNA damage. Required for ATR expression, possibly by stabilizing the protein. Ref.7

Subunit structure: Interacts with ATR

By similarity. Heterodimer with ATR. The heterodimer binds the RPA complex and is then recruited to single-stranded DNA. Interacts with CEP164 (via N-terminus). Interacts with CINP. Ref.1 Ref.7 Ref.10 Ref.13

Subcellular location: Nucleus. Note: Redistributes to discrete nuclear foci upon DNA damage. Ref.1 Ref.10

Tissue specificity: Ubiquitous. Ref.1

Domain: The EEXXXDDL motif is required for the interaction with catalytic subunit PRKDC and its recruitment to sites of DNA damage. Ref.8

Post-translational modification: Phosphorylated by ATR. Ref.1

Sequence similarities: Belongs to the ATRIP family.

Caution: The gene for this protein is either identical to or adjacent to that of TREX1. Some of the mRNAs that encode ATRIP also encode TREX1 in another reading frame.

Sequence caution: The sequence BAB14029.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence BAB14029.1 differs from that shown. Reason: Frameshift at position 650. The sequence BAF84257.1 differs from that shown. Reason: Erroneous initiation.