Rabbit anti-Human, Mouse DUS4L Polyclonal Antibody | anti-DUS4L antibody

DUS4L, ID (DUS4L, tRNA-dihydrouridine(20a/20b) synthase [NAD(P)+]-like, pp35, tRNA-dihydrouridine synthase 4-like) (Azide free) (HRP)

Cat. Num. AAA6293756

• Gene Names: DUS4L; DUS4; PP35
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: DUS4L; Polyclonal Antibody; ID (DUS4L; tRNA-dihydrouridine(20a/20b) synthase [NAD(P)+]-like; pp35; tRNA-dihydrouridine synthase 4-like) (Azide free) (HRP); tRNA-dihydrouridine synthase 4-like; anti-DUS4L antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
• Sequence Length: 317

• Applicable Applications for anti-DUS4L antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: DUS4L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 141-170 amino acids from the Central region of human DUS4L.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(DUS4L Antibody (Center) western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the DUS4L antibody detected the DUS4L protein (arrow).)

Western Blot (WB)

(DUS4L Antibody (Center) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the DUS4L antibody detected the DUS4L protein (arrow).)

Related Product Information for anti-DUS4L antibody

Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs (By similarity).

Product Categories/Family for anti-DUS4L antibody

Antibodies; Enzymes; Synthase

References

Davila, S., et al. Genes Immun. 11(3):232-238(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 31742496
• NCBI GeneID: 11062
• NCBI Accession #: NP_853559.1
• NCBI GenBank Nucleotide #: NP_853559.1
• UniProt Accession #: O95620
• NCBI Official Full Name: tRNA-dihydrouridine(20a/20b) synthase
• NCBI Official Synonym Full Names: dihydrouridine synthase 4 like
• NCBI Official Symbol: DUS4L
• NCBI Official Synonym Symbols: DUS4; PP35
• NCBI Protein Information: tRNA-dihydrouridine(20a/20b) synthase [NAD(P)+]-like
• UniProt Protein Name: tRNA-dihydrouridine(20a/20b) synthase [NAD(P)+]-like
• UniProt Gene Name: DUS4L
• UniProt Entry Name: DUS4L_HUMAN

Uniprot Description

DUS4L: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs. Belongs to the dus family. Dus4 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Oxidoreductase; EC 1.3.1.-; EC 1.-.-.-

Chromosomal Location of Human Ortholog: 7q22-q31

Molecular Function: FAD binding; tRNA dihydrouridine synthase activity