Rabbit anti-Human ROBLD3 Polyclonal Antibody | anti-ROBLD3 antibody

ROBLD3, ID (LAMTOR2, MAPBPIP, ROBLD3, Ragulator complex protein LAMTOR2, Endosomal adaptor protein p14, Late endosomal/lysosomal Mp1-interacting protein, Late endosomal/lysosomal adaptor and MAPK and MTOR activator 2, Mitogen-activated protein-binding pro

Cat. Num. AAA6343051

• Gene Names: LAMTOR2; p14; ENDAP; ROBLD3; HSPC003; MAPBPIP; MAPKSP1AP; Ragulator2
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ROBLD3; Polyclonal Antibody; ID (LAMTOR2; MAPBPIP; Ragulator complex protein LAMTOR2; Endosomal adaptor protein p14; Late endosomal/lysosomal Mp1-interacting protein; Late endosomal/lysosomal adaptor and MAPK and MTOR activator 2; Mitogen-activated protein-binding pro; LAMTOR2; Mitogen-activated protein-binding protein-interacting protein; Roadblock domain-containing protein 3; anti-ROBLD3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-ROBLD3 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: ROBLD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 40-70 amino acids from the Central region of human ROBLD3.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(ROBLD3 Antibody (Center) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the ROBLD3 antibody detected the ROBLD3 protein (arrow).)

Immunohistochemistry (IHC)

(ROBLD3 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ROBLD3 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-ROBLD3 antibody

The product of this gene is highly conserved with a mouse protein associated with the cytoplasmic face of late endosomes and lysosomes. The mouse protein interacts with MAPK scaffold protein 1, a component of the mitogen-activated protein kinase pathway. In humans, a mutation in this gene has been associated with a primary immunodeficiency syndrome, and suggests a role for this protein in endosomal biogenesis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].

Product Categories/Family for anti-ROBLD3 antibody

Antibodies; Binding Proteins

References

Sancak, Y., et al. Cell 141(2):290-303(2010). Wunderlich, W., et al. J. Cell Biol. 152(4):765-776(2001).

NCBI and Uniprot Product Information

• NCBI GI #: 7661728
• NCBI GeneID: 28956
• NCBI Accession #: NP_054736.1
• NCBI GenBank Nucleotide #: NM_014017.3
• UniProt Accession #: Q9Y2Q5
• NCBI Official Full Name: ragulator complex protein LAMTOR2 isoform 1
• NCBI Official Synonym Full Names: late endosomal/lysosomal adaptor, MAPK and MTOR activator 2
• NCBI Official Symbol: LAMTOR2
• NCBI Official Synonym Symbols: p14; ENDAP; ROBLD3; HSPC003; MAPBPIP; MAPKSP1AP; Ragulator2
• NCBI Protein Information: ragulator complex protein LAMTOR2
• UniProt Protein Name: Ragulator complex protein LAMTOR2
• UniProt Gene Name: LAMTOR2
• UniProt Synonym Gene Names: MAPBPIP; ROBLD3; MAPBP-interacting protein
• UniProt Entry Name: LTOR2_HUMAN

NCBI Description

The product of this gene is highly conserved with a mouse protein associated with the cytoplasmic face of late endosomes and lysosomes. The mouse protein interacts with MAPK scaffold protein 1, a component of the mitogen-activated protein kinase pathway. In humans, a mutation in this gene has been associated with a primary immunodeficiency syndrome, and suggests a role for this protein in endosomal biogenesis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2009]

Uniprot Description

LAMTOR2: Adapter protein that enhances the efficiency of the MAP kinase cascade. Facilitates the activation of MAPKAPK2 . Heterodimer with MAP2K1IP1. Interacts with ERK and MEK1. Defects in ROBLD3 are the cause of immunodeficiency due to defect in MAPBP-interacting protein. This form of primary immunodeficiency syndrome includes congenital neutropenia, partial albinism, short stature and B-cell and cytotoxic T-cell deficiency. Two alternatively spliced isoforms of the human protein have been described.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 1q22

Cellular Component: lysosomal membrane; late endosome

Molecular Function: protein binding; guanyl-nucleotide exchange factor activity; protein complex scaffold

Biological Process: activation of MAPKK activity; cell growth; positive regulation of TOR signaling pathway; positive regulation of GTPase activity

Disease: Immunodeficiency Due To Defect In Mapbp-interacting Protein