Rabbit anti-Human QPCT Polyclonal Antibody | anti-QPCT antibody
QPCT, CT (QPCT, Glutaminyl-peptide cyclotransferase, Glutaminyl cyclase, Glutaminyl-tRNA cyclotransferase, Glutamyl cyclase) (Biotin)
Gene Names
QPCT; QC; GCT; sQC
Reactivity
Human
Applications
Western Blot, ELISA
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
QPCT; Polyclonal Antibody; CT (QPCT; Glutaminyl-peptide cyclotransferase; Glutaminyl cyclase; Glutaminyl-tRNA cyclotransferase; Glutamyl cyclase) (Biotin); Glutamyl cyclase; anti-QPCT antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Applicable Applications for anti-QPCT antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:100-500
ELISA: 1:1,000
Applications are based on unconjugated antibody.
ELISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
QPCT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 314-343 amino acids from the C-terminal region of human QPCT.
Conjugate
Biotin
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(QPCT Antibody (C-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the QPCT antibody detected the QPCT protein (arrow).)
Related Product Information for anti-QPCT antibody
This gene encodes human pituitary glutaminyl cyclase, which is responsible for the presence of pyroglutamyl residues in many neuroendocrine peptides. The amino acid sequence of this enzyme is 86% identical to that of bovine glutaminyl cyclase.
Product Categories/Family for anti-QPCT antibody
References
Morawski, M., et al. Acta Neuropathol. 120(2):195-207(2010). Stephan, A., et al. FEBS J. 276(22):6522-6536(2009). Marroni, F., et al. Circ Cardiovasc Genet 2(4):322-328(2009). Calvaresi, M., et al. Proteins 73(3):527-538(2008). Cynis, H., et al. J. Mol. Biol. 379(5):966-980(2008).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
35,421 Da
NCBI Official Full Name
glutaminyl-peptide cyclotransferase
NCBI Official Synonym Full Names
glutaminyl-peptide cyclotransferase
NCBI Official Symbol
QPCT
NCBI Official Synonym Symbols
QC; GCT; sQC
NCBI Protein Information
glutaminyl-peptide cyclotransferase
UniProt Protein Name
Glutaminyl-peptide cyclotransferase
UniProt Gene Name
QPCT
UniProt Synonym Gene Names
QC; sQC; EC
UniProt Entry Name
QPCT_HUMAN
Uniprot Description
QPCT: Responsible for the biosynthesis of pyroglutamyl peptides. Has a bias against acidic and tryptophan residues adjacent to the N-terminal glutaminyl residue and a lack of importance of chain length after the second residue. Also catalyzes N-terminal pyroglutamate formation. In vitro, catalyzes pyroglutamate formation of N-terminally truncated form of APP amyloid-beta peptides [Glu-3]-beta-amyloid. May be involved in the N-terminal pyroglutamate formation of several amyloid-related plaque-forming peptides. Belongs to the glutaminyl-peptide cyclotransferase family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 2.3.2.5; Secreted; Secreted, signal peptide; Transferase
Chromosomal Location of Human Ortholog: 2p22.2
Molecular Function: glutaminyl-peptide cyclotransferase activity; zinc ion binding
Biological Process: peptidyl-pyroglutamic acid biosynthetic process, using glutaminyl-peptide cyclotransferase; protein modification process
