Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Rabbit anti-Mouse Arachidonate-5-Lipoxygenase (ALOX5) Polyclonal Antibody | anti-ALOX5 antibody
Polyclonal Antibody to Arachidonate-5-Lipoxygenase (ALOX5)
Gene Names
Alox5; 5LO; 5LX; 5-LO; 5-LOX; AI850497; F730011J02
Reactivity
Mouse
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Arachidonate-5-Lipoxygenase (ALOX5); Polyclonal Antibody; Polyclonal Antibody to Arachidonate-5-Lipoxygenase (ALOX5); anti-ALOX5 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against ALOX5. It has been selected for its ability to recognize ALOX5 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSGSEF- LVSEVFG IAMYRQLPAV HPLFKLLVAH VRFTIAINTK AREQLICEYG LFDKANATGG GGHVQMVQRA VQDLTYSSLC FPEAIKARGM DSTEDIPFYF YRDDGLLVWE AIQSFTMEVV SIYYENDQVV EEDQELQDFV KDVYVYGMRG KKASGFPKSI KSREKL
MGHHHHHHSGSEF- LVSEVFG IAMYRQLPAV HPLFKLLVAH VRFTIAINTK AREQLICEYG LFDKANATGG GGHVQMVQRA VQDLTYSSLC FPEAIKARGM DSTEDIPFYF YRDDGLLVWE AIQSFTMEVV SIYYENDQVV EEDQELQDFV KDVYVYGMRG KKASGFPKSI KSREKL
Sequence Length
674
Applicable Applications for anti-ALOX5 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant ALOX5 (Leu374~Leu536) expressed in E.coli.
Cross Reactivity
Mouse
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2054254
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DABstainingonIHC-P.Samples:MouseTissue))
Immunohistochemistry (IHC)
(DABstainingonIHC-P.Samples:MouseTissue))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
77,967 Da
NCBI Official Full Name
arachidonate 5-lipoxygenase
NCBI Official Synonym Full Names
arachidonate 5-lipoxygenase
NCBI Official Symbol
Alox5
NCBI Official Synonym Symbols
5LO; 5LX; 5-LO; 5-LOX; AI850497; F730011J02
NCBI Protein Information
arachidonate 5-lipoxygenase
UniProt Protein Name
Arachidonate 5-lipoxygenase
Protein Family
UniProt Gene Name
Alox5
UniProt Synonym Gene Names
5-LO; 5-lipoxygenase
Uniprot Description
5-LO: arachidonate 5-lipoxygenase. Arachidonic acid promotes its phosphorylation by p38 MAPK-regulated MAPKAP kinases. Upregulated in glioblastoma multiforme and colon, pancreatic and bladder cancer.
Protein type: EC 1.13.11.34; Lipid Metabolism - arachidonic acid; Lipid Metabolism - linoleic acid; Nuclear envelope; Oxidoreductase
Chromosomal Location of Human Ortholog: 6 E3|6 53.79 cM
Cellular Component: cytoplasm; cytosol; dendrite; extracellular space; membrane; nuclear envelope; nuclear envelope lumen; nuclear matrix; nuclear membrane; nucleoplasm; nucleus; sarcolemma
Molecular Function: arachidonate 5-lipoxygenase activity; dioxygenase activity; iron ion binding; metal ion binding; oxidoreductase activity; oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen; protein binding
Biological Process: acute inflammatory response; arachidonic acid metabolic process; inflammatory response; leukotriene biosynthetic process; leukotriene metabolic process; leukotriene production involved in inflammatory response; lipoxygenase pathway; positive regulation of vasoconstriction; sensory perception of pain
Research Articles on ALOX5
Similar Products
Product Notes
The ALOX5 alox5 (Catalog #AAA2001385) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Arachidonate-5-Lipoxygenase (ALOX5) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Arachidonate-5-Lipoxygenase (ALOX5) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the ALOX5 alox5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSG SEF- LVSEVFG IAMYRQLPAV HPLFKLLVAH VRFTIAINTK AREQLICEYG LFDKANATGG GGHVQMVQRA VQDLTYSSLC FPEAIKARGM DSTEDIPFYF YRDDGLLVWE AIQSFTMEVV SIYYENDQVV EEDQELQDFV KDVYVYGMRG KKASGFPKSI KSREKL. It is sometimes possible for the material contained within the vial of "Arachidonate-5-Lipoxygenase (ALOX5), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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