Rabbit anti-Human PPP2R1A Polyclonal Antibody | anti-PPP2R1A antibody

PPP2R1A, CT (PPP2R1A, Serine/threonine-protein phosphatase 2A 65kD regulatory subunit A alpha isoform, Medium tumor antigen-associated 61kD protein, PP2A subunit A isoform PR65-alpha, PP2A subunit A isoform R1-alpha) (APC)

Cat. Num. AAA6336644

• Gene Names: PPP2R1A; MRD36; PR65A; PP2AAALPHA; PP2A-Aalpha
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PPP2R1A; Polyclonal Antibody; CT (PPP2R1A; Serine/threonine-protein phosphatase 2A 65kD regulatory subunit A alpha isoform; Medium tumor antigen-associated 61kD protein; PP2A subunit A isoform PR65-alpha; PP2A subunit A isoform R1-alpha) (APC); PP2A subunit A isoform R1-alpha; anti-PPP2R1A antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-PPP2R1A antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: PPP2R1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 454-481 amino acids from the C-terminal region of human PPP2R1A.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(PPP2R1A Antibody (C-term) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the PPP2R1A antibody detected the PPP2R1A protein (arrow).)

Related Product Information for anti-PPP2R1A antibody

This gene encodes a constant regulatory subunit of protein phosphatase 2. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The constant regulatory subunit A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. This gene encodes an alpha isoform of the constant regulatory subunit A. Alternatively spliced transcript variants have been described. [provided by RefSeq].

Product Categories/Family for anti-PPP2R1A antibody

Antibodies; Enzymes; Phosphatase

References

Jones, S., et al. Science 330(6001):228-231(2010). Schmitz, M.H., et al. Nat. Cell Biol. 12(9):886-893(2010). Heikkinen, P.T., et al. J. Biol. Chem. 285(6):3740-3749(2010). Dupont, W.D., et al. Cancer 116(1):8-19(2010). Wang, Q., et al. Neoplasia 11(10):1012-1021(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 21361399
• NCBI GeneID: 5518
• NCBI Accession #: NP_055040.2
• NCBI GenBank Nucleotide #: NM_014225.5
• UniProt Accession #: P30153; Q13773; Q6ICQ3; Q96DH3
• Molecular Weight: 65,309 Da
• NCBI Official Full Name: serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform
• NCBI Official Synonym Full Names: protein phosphatase 2 regulatory subunit A, alpha
• NCBI Official Symbol: PPP2R1A
• NCBI Official Synonym Symbols: MRD36; PR65A; PP2AAALPHA; PP2A-Aalpha
• NCBI Protein Information: serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform
• UniProt Protein Name: Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform
• UniProt Gene Name: PPP2R1A
• UniProt Entry Name: 2AAA_HUMAN

Uniprot Description

PPP2R1A: The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. Required for proper chromosome segregation and for centromeric localization of SGOL1 in mitosis. Found in a complex with at least ARL2, PPP2CB, PPP2R1A, PPP2R2A, PPP2R5E and TBCD. PP2A consists of a common heterodimeric core enzyme, composed of PPP2CA a 36 kDa catalytic subunit (subunit C) and PPP2R1A a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules. Interacts with IPO9. Interacts with TP53 and SGOL1. Interacts with PLA2G16; this interaction might decrease PP2A activity. Belongs to the phosphatase 2A regulatory subunit A family.

Protein type: Motility/polarity/chemotaxis; Protein phosphatase, regulatory subunit

Chromosomal Location of Human Ortholog: 19q13.41

Cellular Component: chromosome, pericentric region; cytosol; protein phosphatase type 2A complex

Molecular Function: antigen binding; protein binding; protein heterodimerization activity; protein phosphatase regulator activity

Biological Process: apoptosis; chromosome segregation; G2/M transition of mitotic cell cycle; mitotic nuclear envelope reassembly; mRNA catabolic process, nonsense-mediated decay; protein amino acid dephosphorylation; protein complex assembly

Disease: Mental Retardation, Autosomal Dominant 36