Rabbit anti-Human MYT1 Polyclonal Antibody | anti-MYT1 antibody

MYT1, CT (Membrane-associated Tyrosine-and Threonine-specific cdc2-inhibitory Kinase, Myt1 Kinase, PKMYT1) (Azide free) (HRP)

Cat. Num. AAA6489059

• Gene Names: PKMYT1; MYT1; PPP1R126
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: MYT1; Polyclonal Antibody; CT (Membrane-associated Tyrosine-and Threonine-specific cdc2-inhibitory Kinase; Myt1 Kinase; PKMYT1) (Azide free) (HRP); anti-MYT1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PKMYT1.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-MYT1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-1:500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human PKMYT1 (KLH).
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-PKMYT1 Pabin A375(left) and Y79 (right)cell line lysate. PKMYT1(arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of PKMYT1 (arrow) using rabbit polyclonal PKMYT1 C-term. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PKMYT1 gene.)

Product Categories/Family for anti-MYT1 antibody

Antibodies; Tyrosine Kinases

References

1. Dai, X., et al., J. Invest. Dermatol. 122(6):1356-1364 (2004). 2. Nakajima, H., et al., J. Biol. Chem. 278(28):25277-25280 (2003). 3. Passer, B.J., et al., Proc. Natl. Acad. Sci. USA 100(5):2284-2289 (2003). 4. Okumura, E., et al., Nat. Cell Biol. 4(2):111-116 (2002). 5. Booher, R.N., et al., J. Biol. Chem. 272(35):22300-22306 (1997).

NCBI and Uniprot Product Information

• NCBI GI #: 33383241
• NCBI GeneID: 9088
• NCBI Accession #: NP_004194
• NCBI GenBank Nucleotide #: NM_004203.4
• UniProt Accession #: Q99640; O14731; Q7LE24; Q8TCM9; B3KUN8; B4DXD4; D3DUA4; F8W164; I3L1V2
• Molecular Weight: 47,284 Da
• NCBI Official Full Name: membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase isoform 1
• NCBI Official Synonym Full Names: protein kinase, membrane associated tyrosine/threonine 1
• NCBI Official Symbol: PKMYT1
• NCBI Official Synonym Symbols: MYT1; PPP1R126
• NCBI Protein Information: membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase
• UniProt Protein Name: Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase
• Protein Family: Myelin transcription factor
• UniProt Gene Name: PKMYT1
• UniProt Synonym Gene Names: MYT1
• UniProt Entry Name: PMYT1_HUMAN

NCBI Description

This gene encodes a member of the serine/threonine protein kinase family. The encoded protein is a membrane-associated kinase that negatively regulates the G2/M transition of the cell cycle by phosphorylating and inactivating cyclin-dependent kinase 1. The activity of the encoded protein is regulated by polo-like kinase 1. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012]

Uniprot Description

Myt1: a dual specificity protein kinase of the WEE family. Preferentially phosphorylates and inactivates cell division cycle 2 protein (CDC2), and thus negatively regulates cell cycle G2/M transition. This kinase is associated with the membrane throughout the cell cycle. Its activity is highly regulated during the cell cycle. Alternative splice-variant isoforms have been reported.

Protein type: EC 2.7.11.1; Kinase, protein; Protein kinase, Other; Protein kinase, dual-specificity (non-receptor); Other group; WEE family

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: nucleoplasm; Golgi membrane; Golgi apparatus; endoplasmic reticulum membrane; membrane; endoplasmic reticulum; cytosol

Molecular Function: protein serine/threonine kinase activity; protein binding; metal ion binding; kinase activity; ATP binding; protein kinase activity

Biological Process: mitosis; regulation of cell cycle; regulation of mitosis; regulation of cyclin-dependent protein kinase activity; mitotic cell cycle; G2/M transition of mitotic cell cycle; protein amino acid phosphorylation; G1/S transition of mitotic cell cycle