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Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Rabbit anti-Human RIO Kinase 1 Polyclonal Antibody | anti-RIOK1 antibody

Polyclonal Antibody to RIO Kinase 1 (RIOK1)

Gene Names
RIOK1; RIO1; AD034; RRP10; bA288G3.1
Reactivity
Human
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
RIO Kinase 1; Polyclonal Antibody; Polyclonal Antibody to RIO Kinase 1 (RIOK1); anti-RIOK1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against RIOK1. It has beenselected for its ability to recognize RIOK1 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Liquid
Concentration
500ug/mL (varies by lot)
Sequence Length
567
Applicable Applications for anti-RIOK1 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western blotting: 0.2-2ug/mL;1:250-2500
Immunohistochemistry: 5-20ug/mL;1:25-100
Immunocytochemistry: 5-20ug/mL;1:25-100
Optimal working dilutions must be determined by end user.
Fragment
RIOK1 (Thr180~Val479)
Organism Species
Homo sapiens (Human)
Cross Reactivity
Human
Conjugate
No Conjugate
Immunogen
Recombinant RIOK1 (Thr180~Val479) expressed in E Coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2085531
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Immunohistochemistry (IHC)

(DABstainingonIHC-P.Samples:HumanTissue))

Immunohistochemistry (IHC) (DABstainingonIHC-P.Samples:HumanTissue))

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
65,583 Da
NCBI Official Full Name
serine/threonine-protein kinase RIO1 isoform 1
NCBI Official Synonym Full Names
RIO kinase 1
NCBI Official Symbol
RIOK1
NCBI Official Synonym Symbols
RIO1; AD034; RRP10; bA288G3.1
NCBI Protein Information
serine/threonine-protein kinase RIO1
UniProt Protein Name
Serine/threonine-protein kinase RIO1
UniProt Gene Name
RIOK1

NCBI Description

The protein encoded by this gene competes with pICln for inclusion in the protein arginine methyltransferase 5 complex. This complex targets substrates for dimethylation. The encoded protein is essential for the last steps in the maturation of 40S subunits. [provided by RefSeq, Jan 2017]

Uniprot Description

RIOK1: This gene includes two alternatively spliced transcript variants, which encode different isoforms. The function of this gene has not been determined. [provided by RefSeq, Jul 2008]

Protein type: ATYPICAL group; EC 2.7.11.1; Kinase, protein; Protein kinase, atypical; RIO family; RIO1 subfamily

Chromosomal Location of Human Ortholog: 6p24.3

Cellular Component: cytosol; nucleolar preribosome, small subunit precursor; nucleoplasm

Molecular Function: ATP binding; hydrolase activity; metal ion binding; protein binding; protein serine/threonine kinase activity

Biological Process: maturation of SSU-rRNA; positive regulation of rRNA processing; protein amino acid phosphorylation; ribosomal small subunit biogenesis and assembly; rRNA processing

Research Articles on RIOK1

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Product Notes

The RIOK1 riok1 (Catalog #AAA2027619) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to RIO Kinase 1 (RIOK1) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RIO Kinase 1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 0.2-2ug/mL;1:250-2500 Immunohistochemistry: 5-20ug/mL;1:25-100 Immunocytochemistry: 5-20ug/mL;1:25-100 Optimal working dilutions must be determined by end user. . Researchers should empirically determine the suitability of the RIOK1 riok1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RIO Kinase 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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