Rabbit anti-Human, Rat PCGF6 Polyclonal Antibody | anti-PCGF6 antibody

PCGF6, ID (PCGF6, MBLR, RNF134, Polycomb group RING finger protein 6, Mel18 and Bmi1-like RING finger, RING finger protein 134) (MaxLight 550)

Cat. Num. AAA6331777

• Gene Names: PCGF6; MBLR; RNF134
• Reactivity: Human, Rat
• Applications: Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: PCGF6; Polyclonal Antibody; ID (PCGF6; MBLR; RNF134; Polycomb group RING finger protein 6; Mel18 and Bmi1-like RING finger; RING finger protein 134) (MaxLight 550); RING finger protein 134; anti-PCGF6 antibody

• Host: Rabbit
• Reactivity: Human, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PCGF6. Species Crossreactivity: rat
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight550.

• Applicable Applications for anti-PCGF6 antibody: Western Blot (WB), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 190-217 in the central region of human PCGF6, UniProt Accession #Q9BYE7. Species sequence homology: mouse
• Conjugate: MaxLight550
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in NCI-H292, rat liver, stomach and testis cell line lysates (35ug/lane) usingMBS6005108. This demonstrates that MBS6005108 detected the PCGF6 protein (arrow).)

Related Product Information for anti-PCGF6 antibody

The protein encoded by this gene contains a RING finger motif, which is most closely related to those of polycomb group (PcG) proteins RNF110/MEL-18 and BMI1. PcG proteins are known to form protein complexes and function as transcription repressors. This protein has been shown to interact with some PcG proteins and act as a transcription repressor. The activity of this protein is found to be regulated by cell cycle dependent phosphorylation. Alternatively spliced transcript variants encoding different isoforms have been identified.

Product Categories/Family for anti-PCGF6 antibody

Antibodies; Transcription Factors; Zinc (Ring) Finger

References

1. Lee, J.Y., et al. Cancer Res. 68(11):4201-4209(2008). 2. Zhang, J., et al. J. Biol. Chem. 283(12):7464-7469(2008). 3. Lee, M.G., et al. Cell 128(5):877-887(2007). 4. Zhong, N., et al. Biochem. Biophys. Res. Commun. 338(2):855-861(2005). 5. Deloukas, P., et al. Nature 429(6990):375-381(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 58761530
• NCBI GeneID: 84108
• NCBI Accession #: NP_001011663.1
• NCBI GenBank Nucleotide #: NM_001011663.1
• UniProt Accession #: Q9BYE7; Q5SYD1; Q5SYD6; Q96ID9; Q96SJ1; A8K3R4
• Molecular Weight: 30,249 Da
• NCBI Official Full Name: polycomb group RING finger protein 6 isoform a
• NCBI Official Synonym Full Names: polycomb group ring finger 6
• NCBI Official Symbol: PCGF6
• NCBI Official Synonym Symbols: MBLR; RNF134
• NCBI Protein Information: polycomb group RING finger protein 6
• UniProt Protein Name: Polycomb group RING finger protein 6
• Protein Family: Polycomb group RING finger protein
• UniProt Gene Name: PCGF6
• UniProt Synonym Gene Names: MBLR; RNF134

NCBI Description

The protein encoded by this gene contains a RING finger motif, which is most closely related to those of polycomb group (PcG) proteins RNF110/MEL-18 and BMI1. PcG proteins are known to form protein complexes and function as transcription repressors. This protein has been shown to interact with some PcG proteins and act as a transcription repressor. The activity of this protein is found to be regulated by cell cycle dependent phosphorylation. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

MBLR: Transcriptional repressor. May modulate the levels of histone H3K4Me3 by activating KDM5D histone demethylase. Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 10q24.33

Cellular Component: nucleoplasm; nucleus; PcG protein complex

Molecular Function: DNA binding; DNA binding transcription factor activity; metal ion binding; protein binding

Biological Process: negative regulation of G0 to G1 transition; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; transcription, DNA-dependent