Rabbit anti-Human, Rat PCGF6 Polyclonal Antibody | anti-PCGF6 antibody

PCGF6, ID (PCGF6, MBLR, RNF134, Polycomb group RING finger protein 6, Mel18 and Bmi1-like RING finger, RING finger protein 134) (FITC)

Cat. Num. AAA6331773

• Gene Names: PCGF6; MBLR; RNF134
• Reactivity: Human, Rat
• Applications: Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: PCGF6; Polyclonal Antibody; ID (PCGF6; MBLR; RNF134; Polycomb group RING finger protein 6; Mel18 and Bmi1-like RING finger; RING finger protein 134) (FITC); RING finger protein 134; anti-PCGF6 antibody

• Host: Rabbit
• Reactivity: Human, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PCGF6. Species Crossreactivity: rat
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-PCGF6 antibody: Western Blot (WB), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 190-217 in the central region of human PCGF6, UniProt Accession #Q9BYE7. Species sequence homology: mouse
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in NCI-H292, rat liver, stomach and testis cell line lysates (35ug/lane) usingMBS6005108. This demonstrates that MBS6005108 detected the PCGF6 protein (arrow).)

Related Product Information for anti-PCGF6 antibody

The protein encoded by this gene contains a RING finger motif, which is most closely related to those of polycomb group (PcG) proteins RNF110/MEL-18 and BMI1. PcG proteins are known to form protein complexes and function as transcription repressors. This protein has been shown to interact with some PcG proteins and act as a transcription repressor. The activity of this protein is found to be regulated by cell cycle dependent phosphorylation. Alternatively spliced transcript variants encoding different isoforms have been identified.

Product Categories/Family for anti-PCGF6 antibody

Antibodies; Transcription Factors; Zinc (Ring) Finger

References

1. Lee, J.Y., et al. Cancer Res. 68(11):4201-4209(2008). 2. Zhang, J., et al. J. Biol. Chem. 283(12):7464-7469(2008). 3. Lee, M.G., et al. Cell 128(5):877-887(2007). 4. Zhong, N., et al. Biochem. Biophys. Res. Commun. 338(2):855-861(2005). 5. Deloukas, P., et al. Nature 429(6990):375-381(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 58761530
• NCBI GeneID: 84108
• NCBI Accession #: NP_001011663.1
• NCBI GenBank Nucleotide #: NM_001011663.1
• UniProt Accession #: Q9BYE7; Q5SYD1; Q5SYD6; Q96ID9; Q96SJ1; A8K3R4
• Molecular Weight: 30,249 Da
• NCBI Official Full Name: polycomb group RING finger protein 6 isoform a
• NCBI Official Synonym Full Names: polycomb group ring finger 6
• NCBI Official Symbol: PCGF6
• NCBI Official Synonym Symbols: MBLR; RNF134
• NCBI Protein Information: polycomb group RING finger protein 6
• UniProt Protein Name: Polycomb group RING finger protein 6
• Protein Family: Polycomb group RING finger protein
• UniProt Gene Name: PCGF6
• UniProt Synonym Gene Names: MBLR; RNF134

NCBI Description

The protein encoded by this gene contains a RING finger motif, which is most closely related to those of polycomb group (PcG) proteins RNF110/MEL-18 and BMI1. PcG proteins are known to form protein complexes and function as transcription repressors. This protein has been shown to interact with some PcG proteins and act as a transcription repressor. The activity of this protein is found to be regulated by cell cycle dependent phosphorylation. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

MBLR: Transcriptional repressor. May modulate the levels of histone H3K4Me3 by activating KDM5D histone demethylase. Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 10q24.33

Cellular Component: nucleoplasm; nucleus; PcG protein complex

Molecular Function: DNA binding; DNA binding transcription factor activity; metal ion binding; protein binding

Biological Process: negative regulation of G0 to G1 transition; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; transcription, DNA-dependent