Rabbit anti-Human, Mouse OTUD6B Polyclonal Antibody | anti-OTUD6B antibody

OTUD6B, CT (OTUD6B, DUBA5, OTU domain-containing protein 6B, DUBA-5) (Azide free) (HRP)

Cat. Num. AAA6329937

• Gene Names: OTUD6B; DUBA5; CGI-77; DUBA-5
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: OTUD6B; Polyclonal Antibody; CT (OTUD6B; DUBA5; OTU domain-containing protein 6B; DUBA-5) (Azide free) (HRP); DUBA-5; anti-OTUD6B antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human OTUD6B. Species Crossreactivity: mouse
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-OTUD6B antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide corresponding to a region between a portion within aa241-270 from the C-terminal region of human OTUD6B, conjugated to KLH.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(OTUD6B Antibody (C-term) western blot analysis in mouse cerebellum tissue lysates (15ug/lane).This demonstrates the OTUD6B antibody detected OTUD6B protein (arrow).)

Flow Cytometry (FC/FACS)

(OTUD6B Antibody (C-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-OTUD6B antibody

Antibodies; Proteins

References

1. Kimura, K., et al. Genome Res. 16(1):55-65(2006). 2. Gerhard, D.S., et al. Genome Res. 14 (10B), 2121-2127 (2004).

NCBI and Uniprot Product Information

• NCBI GI #: 157364937
• NCBI GeneID: 51633
• NCBI Accession #: NP_057107.3
• NCBI GenBank Nucleotide #: NM_016023.3
• UniProt Accession #: Q8N6M0; Q9NTA4; Q9Y387; A8K6I1; B4DEY0
• Molecular Weight: 33813
• NCBI Official Full Name: OTU domain-containing protein 6B isoform 1
• NCBI Official Synonym Full Names: OTU domain containing 6B
• NCBI Official Symbol: OTUD6B
• NCBI Official Synonym Symbols: DUBA5; CGI-77; DUBA-5
• NCBI Protein Information: OTU domain-containing protein 6B
• UniProt Protein Name: OTU domain-containing protein 6B
• Protein Family: OTU domain-containing protein
• UniProt Gene Name: OTUD6B
• UniProt Synonym Gene Names: DUBA5
• UniProt Entry Name: OTU6B_HUMAN

NCBI Description

This gene encodes a member of the ovarian tumor domain (OTU)-containing subfamily of deubiquitinating enzymes. Deubiquitinating enzymes are primarily involved in removing ubiquitin from proteins targeted for degradation. This protein may function as a negative regulator of the cell cycle in B cells. [provided by RefSeq, Nov 2013]