Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Transforming protein RhoA (RHOA) Standard Curve.)
Rat Transforming Protein RhoA (RHOA) ELISA Kit | RHOA elisa kit
Rat Transforming Protein RhoA (RHOA) ELISA Kit
Gene Names
Rhoa; Arha; Arha2
Reactivity
Rat
Synonyms
Transforming Protein RhoA (RHOA); Rat Transforming Protein RhoA (RHOA) ELISA Kit; RHOA; ARH12; ARHA; RHO12; RHOH12; Aplysia ras-related homolog 12; oncogene RHO H12; small GTP binding protein RhoA; RHOA elisa kit
Reactivity
Rat
Specificity
Rat Transforming protein RhoA (RHOA) ELISA Kit has high sensitivity and excellent specificity for detection of Rat RHOA. No significant cross-reactivity or interference between Rat RHOA and analogues was observed.
Sequence Length
193
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Transforming protein RhoA (RHOA) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Transforming protein RhoA (RHOA) Standard Curve.)
Related Product Information for RHOA elisa kit
Description: This Rat Transforming protein RhoA (RHOA) ELISA Kit employs a two-site sandwich ELISA to quantitate RHOA in samples. An antibody specific for RHOA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any RHOA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RHOA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RHOA bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: RhoA is a small GTPase protein known to regulate the actin cytoskeleton in the formation of stress fibers. It acts upon two known effector proteins: ROCK1 and DIAPH1. RhoA is part of a larger family of related proteins known as the Ras superfamily; proteins involved in the regulation and timing of cell division.RhoA activates ROCK (RhoA kinase) which stimulates LIM kinase, which then stimulates Cofilin, which effectively re-organises the Actin cytoskeleton of the cell. In the case of neurons, activation of this pathway results in growth cone collapse, therefore inhibits the growth and repair of neural pathways and axons. Inhibition of this pathway by its various components usually results in some level of improved remyelination.
Background: RhoA is a small GTPase protein known to regulate the actin cytoskeleton in the formation of stress fibers. It acts upon two known effector proteins: ROCK1 and DIAPH1. RhoA is part of a larger family of related proteins known as the Ras superfamily; proteins involved in the regulation and timing of cell division.RhoA activates ROCK (RhoA kinase) which stimulates LIM kinase, which then stimulates Cofilin, which effectively re-organises the Actin cytoskeleton of the cell. In the case of neurons, activation of this pathway results in growth cone collapse, therefore inhibits the growth and repair of neural pathways and axons. Inhibition of this pathway by its various components usually results in some level of improved remyelination.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
transforming protein RhoA
NCBI Official Synonym Full Names
ras homolog family member A
NCBI Official Symbol
Rhoa
NCBI Official Synonym Symbols
Arha; Arha2
NCBI Protein Information
transforming protein RhoA
UniProt Protein Name
Transforming protein RhoA
Protein Family
UniProt Gene Name
Rhoa
UniProt Synonym Gene Names
Arha; Arha2
UniProt Entry Name
RHOA_RAT
Research Articles on RHOA
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Product Notes
The Rat RHOA rhoa (Catalog #AAA9711920) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9711920 ELISA Kit recognizes Rat RHOA. It is sometimes possible for the material contained within the vial of "Transforming Protein RhoA (RHOA), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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