Rabbit anti-Human, Mouse RAD52 Polyclonal Antibody | anti-RAD52 antibody

Rabbit Polyclonal (IgG) to Human RAD52

Cat. Num. AAA243516

• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: RAD52; Polyclonal Antibody; Rabbit Polyclonal (IgG) to Human RAD52; Anti-RAD52 Antibody (aa70-119) IHC-plus; RAD52 (S. cerevisiae) homolog; RAD52 homolog (S. cerevisiae); Recombination protein RAD52; Human RAD52; anti-RAD52 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: RAD52 Antibody detects endogenous levels of total RAD52 protein.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 418

• Applicable Applications for anti-RAD52 antibody: Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:10000), IF (1:100 - 1:500), IHC-P (10 ug/ml), WB (1:500 - 1:1000)
• Target Species: Human
• Immunogen Description: Synthetic peptide from human RAD52 (aa70-119).
• Immunogen: RAD52 antibody was raised against synthetic peptide from human RAD52 (aa70-119).
• Antigen Modification: aa70-119
• Preparation and Storage: Long term: -20 degree C; Short term: +4 degree C; Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Anti-RAD52 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence of COS7 cells, using RAD52 Antibody. The picture on the right is treated with the synthesized peptide.)

Western Blot (WB)

(Western blot of extracts from 293 cells, using RAD52 Antibody. The lane on the right is treated with the synthesized peptide.)

NCBI and Uniprot Product Information

• NCBI GI #: 109637798
• NCBI GeneID: 5893
• NCBI Accession #: NP_602296.2
• NCBI GenBank Nucleotide #: NM_134424.3
• UniProt Accession #: P43351; Q13205; Q9Y5T7; Q9Y5T8; Q9Y5T9
• Molecular Weight: 12,943 Da
• NCBI Official Full Name: DNA repair protein RAD52 homolog isoform a
• NCBI Official Synonym Full Names: RAD52 homolog (S. cerevisiae)
• NCBI Official Symbol: RAD52
• NCBI Protein Information: DNA repair protein RAD52 homolog; recombination protein RAD52; rhabdomyosarcoma antigen MU-RMS-40.23
• UniProt Protein Name: DNA repair protein RAD52 homolog
• Protein Family: RAD52 motif-containing protein
• UniProt Gene Name: RAD52
• UniProt Entry Name: RAD52_HUMAN

NCBI Description

The protein encoded by this gene shares similarity with Saccharomyces cerevisiae Rad52, a protein important for DNA double-strand break repair and homologous recombination. This gene product was shown to bind single-stranded DNA ends, and mediate the DNA-DNA interaction necessary for the annealing of complementary DNA strands. It was also found to interact with DNA recombination protein RAD51, which suggested its role in RAD51 related DNA recombination and repair. A pseudogene of this gene is present on chromosome 2. Alternative splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Jul 2014]

Uniprot Description

RAD52: a protein involved in DNA double strand breaks and recombination. Associates with and is phosphorylated by c-Abl. Belongs to the RAD52/RAD22 family.

Protein type: DNA repair, damage

Chromosomal Location of Human Ortholog: 12p13-p12.2

Cellular Component: nucleoplasm; nucleus

Molecular Function: identical protein binding; protein binding; DNA binding

Biological Process: DNA recombinase assembly; double-strand break repair; DNA repair; double-strand break repair via homologous recombination; DNA recombination