Rabbit anti-Human, Mouse OTUD6B Polyclonal Antibody | anti-OTUD6B antibody

OTUD6B, CT (OTUD6B, DUBA5, OTU domain-containing protein 6B, DUBA-5) (FITC)

Cat. Num. AAA6329936

• Gene Names: OTUD6B; DUBA5; CGI-77; DUBA-5
• Reactivity: Human, Mouse
• Applications: Flow Cytometry, Functional Assay, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: OTUD6B; Polyclonal Antibody; CT (OTUD6B; DUBA5; OTU domain-containing protein 6B; DUBA-5) (FITC); DUBA-5; anti-OTUD6B antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human OTUD6B. Species Crossreactivity: mouse
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-OTUD6B antibody: Flow Cytometry (FC/FACS), Western Blot (WB), FLISA
• Application Notes: FC/FACS: 1:10-1:50; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide corresponding to a region between a portion within aa241-270 from the C-terminal region of human OTUD6B, conjugated to KLH.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(OTUD6B Antibody (C-term) western blot analysis in mouse cerebellum tissue lysates (15ug/lane).This demonstrates the OTUD6B antibody detected OTUD6B protein (arrow).)

Flow Cytometry (FC/FACS)

(OTUD6B Antibody (C-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-OTUD6B antibody

Antibodies; Proteins

References

1. Kimura, K., et al. Genome Res. 16(1):55-65(2006). 2. Gerhard, D.S., et al. Genome Res. 14 (10B), 2121-2127 (2004).

NCBI and Uniprot Product Information

• NCBI GI #: 157364937
• NCBI GeneID: 51633
• NCBI Accession #: NP_057107.3
• NCBI GenBank Nucleotide #: NM_016023.3
• UniProt Accession #: Q8N6M0; Q9NTA4; Q9Y387; A8K6I1; B4DEY0
• Molecular Weight: 33813
• NCBI Official Full Name: OTU domain-containing protein 6B isoform 1
• NCBI Official Synonym Full Names: OTU domain containing 6B
• NCBI Official Symbol: OTUD6B
• NCBI Official Synonym Symbols: DUBA5; CGI-77; DUBA-5
• NCBI Protein Information: OTU domain-containing protein 6B
• UniProt Protein Name: OTU domain-containing protein 6B
• Protein Family: OTU domain-containing protein
• UniProt Gene Name: OTUD6B
• UniProt Synonym Gene Names: DUBA5
• UniProt Entry Name: OTU6B_HUMAN

NCBI Description

This gene encodes a member of the ovarian tumor domain (OTU)-containing subfamily of deubiquitinating enzymes. Deubiquitinating enzymes are primarily involved in removing ubiquitin from proteins targeted for degradation. This protein may function as a negative regulator of the cell cycle in B cells. [provided by RefSeq, Nov 2013]