Rabbit anti-Human, Mouse Insulin-Like Growth Factor I Receptor Monoclonal Antibody | anti-IGF1R antibody

Insulin-Like Growth Factor I Receptor, phosphorylated (Tyr1135/1136) Insulin Receptor (Tyr1150/1151) (IGF1R)

Cat. Num. AAA632078

• Gene Names: IGF1R; IGFR; CD221; IGFIR; JTK13; MGC18216; MGC142170; MGC142172
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Serum; Serum
• Synonyms: Insulin-Like Growth Factor I Receptor; Monoclonal Antibody; phosphorylated (Tyr1135/1136) Insulin Receptor (Tyr1150/1151) (IGF1R); Anti -Insulin-Like Growth Factor I Receptor; anti-IGF1R antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: 5i120
• Specificity: Detects endogenous levels of IGF-I receptor and insulin receptor only when phosphorylated at tyrosine1135/1136 or tyrosine 1150/1151, respectively. It does not cross-react with other related tyrosine-phosphorylated tyrosine kinases.
• Purity/Purification: Serum; Serum
• Form/Format: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM NaCl, 0.1mg/ml BSA, 50% glycerol.

• Applicable Applications for anti-IGF1R antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 1:1000
• Immunogen: Synthetic phosphopeptide (KLH-coupled) corresponding to residues surrounding Tyr1135/1136 of human IGF-I receptor.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot. Store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(MBS632078 specifically binds to tyrosine phosphorylated IGF-I and insulin receptors, but not other phosphorylated tyrosine kinases. Western blot analysis of of extracts from cells expressing different activated tyrosine kinase proteins, using MBS632078 (upper) or Phospho-Tyrosine (lower).)

Western Blot (WB)

(Western blot analysis of untreated and IGF-treated HeLa cell extracts as well as untreated and insulin-treated H-4-II-E cell extracts using MBS632078.)

Related Product Information for anti-IGF1R antibody

Type I insulin-like growth factor receptor IGF-IR), a transmembrane tyrosine kinase, is widely expressed among many cell types in fetal and postnatal tissues, as well as many cell lines (1-3). Upon binding of its ligands, IGF-I and IGF-II, autophosphorylation of receptors occurs. The triple tyrosine cluster (Tyr1131, Tyr1135 and Tyr1136) within the kinase domain is the earliest major site of autophosphorylation (4). Phosphorylation of these three tyrosine residues is necessary

Product Categories/Family for anti-IGF1R antibody

Antibodies; Abs to Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 4557665
• NCBI GeneID: 3480
• NCBI Accession #: NP_000866.1
• NCBI GenBank Nucleotide #: NM_000875.3
• UniProt Accession #: P08069; Q14CV2; Q9UCC0; B1B5Y2; O43467; Q3B833
• Molecular Weight: 154,793 Da
• NCBI Official Full Name: insulin-like growth factor 1 receptor
• NCBI Official Synonym Full Names: insulin-like growth factor 1 receptor
• NCBI Official Symbol: IGF1R
• NCBI Official Synonym Symbols: IGFR; CD221; IGFIR; JTK13; MGC18216; MGC142170; MGC142172
• NCBI Protein Information: insulin-like growth factor 1 receptor; IGF-I receptor; OTTHUMP00000194784; soluble IGF1R variant 1; soluble IGF1R variant 2; insulin-like growth factor I receptor
• UniProt Protein Name: Insulin-like growth factor 1 receptor
• Protein Family: Insulin-like growth factor 1 receptor
• UniProt Gene Name: IGF1R
• UniProt Entry Name: IGF1R_HUMAN

NCBI Description

This receptor binds insulin-like growth factor with a high affinity. It has tyrosine kinase activity. The insulin-like growth factor I receptor plays a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival. [provided by RefSeq]

Uniprot Description

IGF1R: a receptor tyrosine kinase that binds insulin-like growth factor 1 (IGF1) with a high affinity and IGF2 with a lower affinity. Functions as an anti-apoptotic agent by enhancing cell survival. Can play a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. Tetramer of 2 alpha and 2 beta chains linked by disulfide bonds. The alpha chains contribute to the formation of the ligand- binding domain, while the beta chain carries the kinase domain. Interacts with PIK3R1 and with the PTB/PID domains of IRS1 and SHC1 in vitro when autophosphorylated on tyrosine residues. Mutated in rare cases of pre- and post-natal growth retardation. One SNP associated with increased human longevity. Increased expression of IGF1R and other pathway members associated with progression and malignancy in a range of cancers. Inhibitors: AG1024, AEW541.

Protein type: Kinase, protein; Protein kinase, TK; EC 2.7.10.1; Protein kinase, tyrosine (receptor); Membrane protein, integral; TK group; InsR family

Chromosomal Location of Human Ortholog: 15q26.3

Cellular Component: neuron projection; membrane; intracellular membrane-bound organelle; integral to plasma membrane; plasma membrane; caveola; receptor complex

Molecular Function: insulin binding; insulin-like growth factor binding; identical protein binding; protein binding; insulin-like growth factor I binding; insulin receptor substrate binding; protein-tyrosine kinase activity; G-protein alpha-subunit binding; phosphoinositide 3-kinase binding; insulin receptor binding; ATP binding; insulin-like growth factor receptor activity

Biological Process: epidermis development; phosphoinositide 3-kinase cascade; inactivation of MAPKK activity; protein heterooligomerization; protein amino acid autophosphorylation; exocrine pancreas development; signal transduction; regulation of JNK cascade; response to vitamin E; positive regulation of MAPKKK cascade; mammary gland development; male sex determination; positive regulation of cell proliferation; protein tetramerization; positive regulation of cytokinesis; phosphoinositide-mediated signaling; negative regulation of protein kinase B signaling cascade; positive regulation of mitosis; negative regulation of transcription factor activity; positive regulation of protein kinase B signaling cascade; axonogenesis; insulin-like growth factor receptor signaling pathway; establishment of cell polarity; insulin receptor signaling pathway; immune response; brain development; positive regulation of DNA replication; positive regulation of cell migration; negative regulation of apoptosis

Disease: Insulin-like Growth Factor I, Resistance To