Western Blot (WB)
(METTL2 Antibody (C-term) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the METTL2 antibody detected the METTL2 protein (arrow).)
Rabbit anti-Human METTL2 Polyclonal Antibody | anti-METTL2 antibody
METTL2, CT (METTL2A, METTL2, Methyltransferase-like protein 2A) (Biotin)
Gene Names
METTL2A; METTL2
Reactivity
Human
Applications
Immunofluorescence, ELISA, Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
METTL2; Polyclonal Antibody; CT (METTL2A; Methyltransferase-like protein 2A) (Biotin); METTL2A; Methyltransferase-like protein 2A; anti-METTL2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Applicable Applications for anti-METTL2 antibody
Immunofluorescence (IF), ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
IF: 1:10-50
ELISA: 1:1,000
IHC: 1:50-100
WB: 1:100-500
Applications are based on unconjugated antibody.
ELISA: 1:1,000
IHC: 1:50-100
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
METTL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 336-367 amino acids from the C-terminal region of human METTL2.
Conjugate
Biotin
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(METTL2 Antibody (C-term) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the METTL2 antibody detected the METTL2 protein (arrow).)
Western Blot (WB)
(METTL2 Antibody (C-term) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the METTL2 antibody detected the METTL2 protein (arrow).)
Immunohistochemistry (IHC)
(METTL2 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of METTL2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(METTL2 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of METTL2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of METTL2 Antibody (C-term) with ZR-75-1 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of METTL2 Antibody (C-term) with ZR-75-1 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
Related Product Information for anti-METTL2 antibody
METTL2A is probable methyltransferase (By similarity).
Product Categories/Family for anti-METTL2 antibody
References
Zody, M.C., et al. Nature 440(7087):1045-1049(2006).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
43,537 Da
NCBI Official Full Name
methyltransferase-like protein 2A
NCBI Official Synonym Full Names
methyltransferase like 2A
NCBI Official Symbol
METTL2A
NCBI Official Synonym Symbols
METTL2
NCBI Protein Information
methyltransferase-like protein 2A
UniProt Protein Name
Methyltransferase-like protein 2A
Protein Family
UniProt Gene Name
METTL2A
UniProt Synonym Gene Names
METTL2
UniProt Entry Name
MET2A_HUMAN
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Product Notes
The METTL2 mettl2a (Catalog #AAA6320406) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The METTL2, CT (METTL2A, METTL2, Methyltransferase-like protein 2A) (Biotin) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's METTL2 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB). IF: 1:10-50 ELISA: 1:1,000 IHC: 1:50-100 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the METTL2 mettl2a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "METTL2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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