Rabbit anti-Rat CCL3 Polyclonal Antibody | anti-CCL3 antibody

CCL3 (C C Motif Chemokine 3, CCL 3, CCL3_HUMAN, Chemokine C C Motif Ligand 3, Chemokine CC Motif Ligand 3, G0/G1 Switch Regulatory Protein 19 1, G0/G1 Switch Regulatory Protein 19-1, G0S19 1, G0S19 1 Protein, Heparin Binding Chemotaxis Protein, L2G25B, LD

Cat. Num. AAA6460714

• Gene Names: Ccl3; Scya3; MIP-1a
• Reactivity: Rat
• Applications: Western Blot
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: CCL3; Polyclonal Antibody; CCL3 (C C Motif Chemokine 3; CCL 3; CCL3_HUMAN; Chemokine C C Motif Ligand 3; Chemokine CC Motif Ligand 3; G0/G1 Switch Regulatory Protein 19 1; G0/G1 Switch Regulatory Protein 19-1; G0S19 1; G0S19 1 Protein; Heparin Binding Chemotaxis Protein; L2G25B; LD; anti-CCL3 antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes rat CCL3.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight750.

• Applicable Applications for anti-CCL3 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 0.1-0.5ug/ml, the detection limit is ~1ng/lane under reducing conditions.; FLISA: 0.1-0.5ug/ml; Applications are based on unconjugated antibody.
• Immunogen: Recombinant protein corresponding to rat CCL3 expressed in E. coli. (Position: A24-A92). Species sequence homology: human (74%) and mouse (91%)
• Conjugate: MaxLight750
• Note: Preservative Free
• Preparation and Storage: May be stored at -20 degree C. Stable for 12 months after receipt at -20 degree C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Anti-CCL3 antibody, All lanes: Anti-CCL3 at 0.5ug/ml WB: Recombinant Rat CCL3 Protein 0.5ng Predicted band size: 33KD Observed band size: 33KD)

Product Categories/Family for anti-CCL3 antibody

Antibodies; Growth Factors; Cytokines

References

1. Wolpe, S. D.; Cerami, A. Macrophage inflammatory proteins 1 and 2: members of a novel superfamily of cytokines. FASEB J. 3: 2565-2573, 1989. 2. Nakao, M.; Nomiyama, H.; Shimada, K. Structures of human genes coding for cytokine LD78 and their expression. Molec. Cell. Biol. 10: 3646-3658, 1990. 3. Cook, D. N.; Beck, M. A.; Coffman, T. M.; Kirby, S. L.; Sheridan, J. F.; Pragnell, I. B.; Smithies, O. Requirement of MIP-1-alpha for an inflammatory response to viral infection. Science 269: 1583-1585, 1995. 4. Miyazaki, D.; Nakamura, T.; Toda, M.; Cheung-Chau, K.-W.; Richardson, R. M.; Ono, S. J. Macrophage inflammatory protein-1-alpha as a costimulatory signal for mast cell-mediated immediate hypersensitivity reactions. J. Clin. Invest. 115: 434-442, 2005.

NCBI and Uniprot Product Information

• NCBI GI #: 40254794
• NCBI GeneID: 25542
• NCBI Accession #: NP_037157.2
• NCBI GenBank Nucleotide #: NM_013025.2
• UniProt Accession #: P50229; A1EC87
• Molecular Weight: 10,335 Da
• NCBI Official Full Name: C-C motif chemokine 3
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 3
• NCBI Official Symbol: Ccl3
• NCBI Official Synonym Symbols: Scya3; MIP-1a
• NCBI Protein Information: C-C motif chemokine 3; MIP-1-alpha; Macrophage inflammatory protein 1 alpha (Small inducible cytokine A3); macrophage inflammatory protein 1-alpha; small inducible cytokine A3; small-inducible cytokine A3
• UniProt Protein Name: C-C motif chemokine 3
• Protein Family: C-C motif chemokine
• UniProt Gene Name: Ccl3
• UniProt Synonym Gene Names: Mip1a; Scya3; MIP-1-alpha
• UniProt Entry Name: CCL3_RAT

NCBI Description

mediates monocyte and neutrophil chemotaxis; may play a role in the pathogenesis of acute lung injury [RGD, Feb 2006]

Uniprot Description

Monokine with inflammatory and chemokinetic properties. Has chemotactic activity for monocytes, neutrophils, eosinophils, basophils, and lymphocytes. Required for lung TNF-alpha production, neutrophil recruitment and subsequent lung injury and may function as an autocrine mediator for the macrophage production of TNF-alpha which in turn up-regulates vascular adhesion molecules required for neutrophil influx. This protein binds heparin.