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Western Blot (WB) (METTL2 Antibody (C-term) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the METTL2 antibody detected the METTL2 protein (arrow).)

Rabbit METTL2 Polyclonal Antibody | anti-METTL2A antibody

METTL2 Antibody (C-term)

Gene Names
METTL2A; METTL2
Reactivity
Human (Predicted Reactivity: Zebrafish, Bovine, Mouse)
Applications
ELISA, Immunohistochemistry, Immunofluorescence, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
METTL2; Polyclonal Antibody; METTL2 Antibody (C-term); Methyltransferase-like protein 2A; 211-; METTL2A; anti-METTL2A antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Zebrafish, Bovine, Mouse)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This METTL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 329-359 amino acids from the C-terminal region of human METTL2.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.25 (varies by lot)
Sequence Positions
329-359
Sequence Length
242
Applicable Applications for anti-METTL2A antibody
ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF), Western Blot (WB)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(METTL2 Antibody (C-term) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the METTL2 antibody detected the METTL2 protein (arrow).)

Western Blot (WB) (METTL2 Antibody (C-term) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the METTL2 antibody detected the METTL2 protein (arrow).)

Immunohistochemistry (IHC)

(METTL2 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of METTL2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (METTL2 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of METTL2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of METTL2 Antibody (C-term) with ZR-75-1 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Immunofluorescence (IF) (Confocal immunofluorescent analysis of METTL2 Antibody (C-term) with ZR-75-1 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
Related Product Information for anti-METTL2A antibody
METTL2A is probable methyltransferase (By similarity).
Product Categories/Family for anti-METTL2A antibody
References
Zody, M.C., et al. Nature 440(7087):1045-1049(2006)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
43537
NCBI Official Full Name
methyltransferase-like protein 2A
NCBI Official Synonym Full Names
methyltransferase like 2A
NCBI Official Symbol
METTL2A
NCBI Official Synonym Symbols
METTL2
NCBI Protein Information
methyltransferase-like protein 2A
UniProt Protein Name
Methyltransferase-like protein 2A
UniProt Gene Name
METTL2A
UniProt Synonym Gene Names
METTL2
UniProt Entry Name
MET2A_HUMAN

Uniprot Description

METTL2A: Probable methyltransferase. Belongs to the methyltransferase superfamily. METL family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.1.1.-; Methyltransferase, protein arginine, predicted; Methyltransferase

Chromosomal Location of Human Ortholog: 17q23.2

Molecular Function: tRNA (cytosine)-methyltransferase activity

Biological Process: tRNA methylation

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Product Notes

The METTL2A mettl2a (Catalog #AAA9213659) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 329-359. The METTL2 Antibody (C-term) reacts with Human (Predicted Reactivity: Zebrafish, Bovine, Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's METTL2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF), Western Blot (WB). WB~~1:1000. Researchers should empirically determine the suitability of the METTL2A mettl2a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "METTL2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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