Mouse Connexin 32 Monoclonal Antibody | anti-GJB1 antibody

Connexin 32 (CX32, Gap Junction Beta 1 Protein, CXB-1, Gap Junction 28kD Liver Protein)

Cat. Num. AAA631932

• Gene Names: GJB1; CX32
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by affinity chromatography.
• Synonyms: Connexin 32; Monoclonal Antibody; Connexin 32 (CX32; Gap Junction Beta 1 Protein; CXB-1; Gap Junction 28kD Liver Protein); Anti -Connexin 32 (CX32; anti-GJB1 antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 10K172
• Specificity: Recognizes human Connexin 32. Species Crossreactivity: mouse and rat.
• Purity/Purification: Affinity Purified; Purified by affinity chromatography.
• Form/Format: Supplied as a lyophilized powder from 1.2% sodium acetate, 2% BSA, 0.01% sodium azide. Reconstitute with 1ml sterile PBS.

• Applicable Applications for anti-GJB1 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot and Immunohistochemistry.; Dilution: Western Blot: 0.5-1ug/ml detects Connexin 32 in cells and tissues.; Immunohistochemistry (Frozen): 1-2ug/ml detects Connexin 32 in formalin and acetone fixed tissues.
• Immunogen: Synthetic peptide corresponding to aa105-123 from human connexin-32. Conjugated to KLH.
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-GJB1 antibody

Gap junctions are transmembrane channels that serve to directly link neighboring cells by mediating the exchange of lowmolecular weight metabolites, ions, and second messengers. Gap junctions are formed by the interaction of connexons or hemichannels on adjacent cells. The connexon itself is composed of a hexameric assembly of proteins referred to as connexins. Connexins are highly homologous proteins encoded by a multigene family. The connexins exhibit similar structural features which include a cytoplasmic amino terminal region, four transmembrane domains, two extracellular loops, and a carboxy-terminal cytoplasmic tail of varying length. Modulation of gap junction communication can be achieved by multiple mechanisms and can occur very rapidly or over a period of several hours. These mechanisms include alterations in transcription, translation, stability, posttranslational processing (especially phosphorylation), gating, and insertion or removal from the plasma membrane. Interestingly, reduction or alterations in the levels or types of connexin expressed in a given cell type has been found to correlate with tumor progression and metastasis.

Product Categories/Family for anti-GJB1 antibody

Antibodies; Abs to Connexin; Junction Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 45383418
• NCBI GeneID: 378797
• NCBI Accession #: NP_989702.1
• NCBI GenBank Nucleotide #: NM_204371.2
• UniProt Accession #: Q6URH5
• Molecular Weight: 31,787 Da
• NCBI Official Full Name: connexin 32
• NCBI Official Synonym Full Names: gap junction protein, beta 1, 32kDa<
• NCBI Official Symbol: GJB1
• NCBI Official Synonym Symbols: CX32
• NCBI Protein Information: connexin 32
• UniProt Protein Name: Gap junction protein
• Protein Family: Gap junction beta-1 protein
• UniProt Entry Name: Q6URH5_CHICK

Uniprot Description

Function: One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell

By similarity. RuleBase RU000630

Subunit structure: A connexon is composed of a hexamer of connexins

By similarity. RuleBase RU000630

Subcellular location: Membrane; Multi-pass membrane protein

By similarity RuleBase RU000630.

Sequence similarities: Belongs to the connexin family. RuleBase RU000630