Rabbit NHEDC2 Polyclonal Antibody | anti-SLC9B2 antibody

NHEDC2 Antibody (C-term)

Cat. Num. AAA9206121

• Gene Names: SLC9B2; NHA2; NHE10; NHEDC2
• Reactivity: Human (Predicted Reactivity: Mouse)
• Applications: Western Blot, ELISA
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: NHEDC2; Polyclonal Antibody; NHEDC2 Antibody (C-term); Mitochondrial sodium/hydrogen exchanger 9B2; Mitochondrial Na(+)/H(+) exchanger NHA2; Na(+)/H(+) exchanger-like domain-containing protein 2; NHE domain-containing protein 2; Sodium/hydrogen exchanger-like domain-containing protein 2; Solute carrier family 9 subfamily B member 2; SLC9B2; NHA2; anti-SLC9B2 antibody

• Host: Rabbit
• Reactivity: Human (Predicted Reactivity: Mouse)
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This NHEDC2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 458-486 amino acids from the C-terminal region of human NHEDC2.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Sequence Positions: 458-486
• Sequence Length: 454

• Applicable Applications for anti-SLC9B2 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB~~1:1000
• Function: Na(+)/H(+) antiporter that extrudes Na(+) or Li(+) in exchange for external protons across the membrane (PubMed: 18000046, PubMed:28154142, PubMed: 22948142, PubMed: 18508966). Contributes to the regulation of intracellular pH, sodium homeostasis, and cell volume. Plays an important role for insulin secretion and clathrin-mediated endocytosis in beta-cells (By similarity). Involved in sperm motility and fertility (By similarity). It is controversial whether SLC9B2 plays a role in osteoclast differentiation or not (By similarity).
• Cellular Location: Cell membrane; Multi-pass membrane protein. Mitochondrion membrane; Multi-pass membrane protein. Endosome membrane {ECO:0000250|UniProtKB:Q5BKR2}; Multipass membrane protein. Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane {ECO:0000250|UniProtKB:Q5BKR2}; Multi-pass membrane protein {ECO:0000250|UniProtKB:Q5BKR2}. Cell projection, cilium, flagellum membrane {ECO:0000250|UniProtKB:Q5BKR2}; Multi-pass membrane protein. Basolateral cell membrane {ECO:0000250|UniProtKB:Q5BKR2}; Multi-pass membrane protein. Apical cell membrane {ECO:0000250|UniProtKB:Q5BKR2}; Multi-pass membrane protein. Note=Strong colocalization with LAMP1 and TCIRG1 in osteoclasts. In beta-cells colocalizes with RAB4A and SYP. Localizes to the basolateral membrane of polarized osteoclasts. {ECO:0000250|UniProtKB:Q5BKR2}
• Tissue Location: Widely expressed (PubMed:18508966). High levels detected in the distal tubules of the kidney nephron (PubMed:18508966) Detected in red blood cells (at protein level) (PubMed: 18000046, PubMed:18508966).
• Preparation and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(NHEDC2 Antibody (C-term) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the NHEDC2 antibody detected the NHEDC2 protein (arrow).)

Related Product Information for anti-SLC9B2 antibody

Sodium hydrogen antiporters, such as NHEDC2, convert the proton motive force established by the respiratory chain or the F1F0 mitochondrial ATPase into sodium gradients that drive other energy-requiring processes, transduce environmental signals into cell responses, or function in drug efflux (Xiang et al., 2007 [PubMed 18000046]).

Product Categories/Family for anti-SLC9B2 antibody

Cell Biology; Metabolism; Signal transduction

References

Kalsi, G., et al. Hum. Mol. Genet. 19(12):2497-2506(2010)
Schushan, M., et al. J. Mol. Biol. 396(5):1181-1196(2010)
Fuster, D.G., et al. J. Am. Soc. Nephrol. 19(8):1547-1556(2008)
Battaglino, R.A., et al. Bone 42(1):180-192(2008)
Xiang, M., et al. Proc. Natl. Acad. Sci. U.S.A. 104(47):18677-18681(2007)

NCBI and Uniprot Product Information

• NCBI GI #: 47271479
• NCBI GeneID: 133308
• NCBI Accession #: NP_849155.2
• NCBI GenBank Nucleotide #: NM_178833.5
• UniProt Accession #: Q86UD5; Q6ZMD8; Q96D95; B5ME52
• Molecular Weight: 57564
• NCBI Official Full Name: mitochondrial sodium/hydrogen exchanger 9B2 isoform 1
• NCBI Official Synonym Full Names: solute carrier family 9, subfamily B (NHA2, cation proton antiporter 2), member 2
• NCBI Official Symbol: SLC9B2
• NCBI Official Synonym Symbols: NHA2; NHE10; NHEDC2
• NCBI Protein Information: mitochondrial sodium/hydrogen exchanger 9B2
• UniProt Protein Name: Mitochondrial sodium/hydrogen exchanger 9B2
• Protein Family: Mitochondrial sodium/hydrogen exchanger
• UniProt Gene Name: SLC9B2
• UniProt Synonym Gene Names: NHA2; NHEDC2; NHE domain-containing protein 2
• UniProt Entry Name: SL9B2_HUMAN

NCBI Description

Sodium hydrogen antiporters, such as NHEDC2, convert the proton motive force established by the respiratory chain or the F1F0 mitochondrial ATPase into sodium gradients that drive other energy-requiring processes, transduce environmental signals into cell responses, or function in drug efflux (Xiang et al., 2007 [PubMed 18000046]).[supplied by OMIM, Mar 2008]

Uniprot Description

NHEDC2: Electroneutral exchange of protons for Na(+) and Li(+) across the inner mitochondrial membrane. Contributes to the organellar volume homeostasis. Required for osteoclast differentiation and bone resorption activity. Belongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transporter, ion channel; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 4q24

Cellular Component: mitochondrial inner membrane; integral to membrane

Molecular Function: solute:hydrogen antiporter activity

Biological Process: sodium ion transport; transmembrane transport