Rabbit anti-Human KRTAP1-1 Polyclonal Antibody | anti-KRTAP1-1 antibody

KRTAP1-1, ID (KRTAP1-1, B2A, KAP1.1, KAP1.6, KAP1.7, KRTAP1.1, Keratin-associated protein 1-1, High sulfur keratin-associated protein 1.1, Keratin-associated protein 1.1, Keratin-associated protein 1.6, Keratin-associated protein 1.7) (PE)

Cat. Num. AAA6314853

• Gene Names: KRTAP1-1; HB2A; KAP1.1; KAP1.6; KAP1.7; KAP1.1A; KAP1.1B; KRTAP1A; hKAP1.7; KRTAP1.1
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: KRTAP1-1; Polyclonal Antibody; ID (KRTAP1-1; B2A; KAP1.1; KAP1.6; KAP1.7; KRTAP1.1; Keratin-associated protein 1-1; High sulfur keratin-associated protein 1.1; Keratin-associated protein 1.1; Keratin-associated protein 1.6; Keratin-associated protein 1.7) (PE); Keratin-associated protein 1.7; anti-KRTAP1-1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-KRTAP1-1 antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: KRTAP1-1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 95-125 amino acids from the Central region of human KRTAP1-1.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of KRTAP1-1 Antibody (Center) in K562 cell line lysates (35ug/lane).KRTAP1-1 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(KRTAP1-1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human skin carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the KRTAP1-1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(KRTAP1-1 Antibody (Center) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-KRTAP1-1 antibody

Antibodies; Keratin; Cytokeratin

References

Shimomura, Y., et al. J. Invest. Dermatol. 118(2):226-231(2002). Rogers, M.A., et al. J. Biol. Chem. 276(22):19440-19451(2001).

NCBI and Uniprot Product Information

• NCBI GI #: 13569942
• NCBI GeneID: 81851
• NCBI Accession #: NP_112229.1
• NCBI GenBank Nucleotide #: NM_030967.2
• UniProt Accession #: Q07627; Q96S60; Q96S67; A6NC32
• Molecular Weight: 18,235 Da
• NCBI Official Full Name: keratin-associated protein 1-1
• NCBI Official Synonym Full Names: keratin associated protein 1-1
• NCBI Official Symbol: KRTAP1-1
• NCBI Official Synonym Symbols: HB2A; KAP1.1; KAP1.6; KAP1.7; KAP1.1A; KAP1.1B; KRTAP1A; hKAP1.7; KRTAP1.1
• NCBI Protein Information: keratin-associated protein 1-1; keratin associated protein 1A; keratin associated protein 1.7; keratin-associated protein 1.6; keratin-associated protein 1.7; high sulfur keratin-associated protein 1.1
• UniProt Protein Name: Keratin-associated protein 1-1
• Protein Family: Keratin-associated protein
• UniProt Gene Name: KRTAP1-1
• UniProt Synonym Gene Names: B2A; KAP1.1; KAP1.6; KAP1.7; KRTAP1.1
• UniProt Entry Name: KRA11_HUMAN

NCBI Description

This protein is a member of the keratin-associated protein (KAP) family. The KAP proteins form a matrix of keratin intermediate filaments which contribute to the structure of hair fibers. KAP family members appear to have unique, family-specific amino- and carboxyl-terminal regions and are subdivided into three multi-gene families according to amino acid composition: the high sulfur, the ultrahigh sulfur, and the high tyrosine/glycine KAPs. This protein is a member of the high sulfur KAP family and the gene is localized to a cluster of KAPs at 17q12-q21. [provided by RefSeq, Jul 2008]

Uniprot Description

KRTAP1-1: In the hair cortex, hair keratin intermediate filaments are embedded in an interfilamentous matrix, consisting of hair keratin-associated proteins (KRTAP), which are essential for the formation of a rigid and resistant hair shaft through their extensive disulfide bond cross-linking with abundant cysteine residues of hair keratins. The matrix proteins include the high- sulfur and high-glycine-tyrosine keratins. Belongs to the KRTAP type 1 family. Interacts with hair keratins

Chromosomal Location of Human Ortholog: 17q12

Cellular Component: keratin filament