Mouse anti-Human Keratin, Multi Monoclonal Antibody | anti-KRTAP1-1 antibody

Mouse anti-Human Keratin, Multi (BSA & Azide Free)

Cat. Num. AAA603731

• Gene Names: KRTAP1-1; HB2A; KAP1.1; KAP1.6; KAP1.7; KAP1.1A; KAP1.1B; KRTAP1A; hKAP1.7; KRTAP1.1
• Reactivity: Human
• Applications: Immunohistochemistry, Immunofluorescence
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: Keratin; Multi; Monoclonal Antibody; Mouse anti-Human Keratin; Multi (BSA & Azide Free); Anti -Keratin; anti-KRTAP1-1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 0.N.371
• Specificity: Recognizes keratins 4, 5, 6, 8, 10, 13 and 18. ; Cross-reactivity Reactivity: Human, Bovine, Rat, Mouse and Xenopus.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in 10mM PBS, pH 7.4. Also available with BSA and azide. See MBS603028.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-KRTAP1-1 antibody: Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Recommended Dilution:; Immunohistochemistry (FFPE): 1-2ug/ml Requires HIER using sodium citrate buffer pH6.0; Optimal dilutions to be determined by the researcher.
• Immunogen: Keratin-enriched preparation from cultured human A431 cells.
• Positive Control: Skin, colon carcinoma
• Preparation and Storage: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Immunohistochemistry of formalin-fixed, paraffin-embedded human colon carcinoma using MBS603731 followed by peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of tumor cells.)

Related Product Information for anti-KRTAP1-1 antibody

Applications: Immunofluorescence, Western Blotting, Immunohistochemistry (frozen & formalin/paraffin). Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min., followed by cooling at RT for 20 min.

Product Categories/Family for anti-KRTAP1-1 antibody

Antibodies; Abs to Keratin; Cytokeratin

NCBI and Uniprot Product Information

• NCBI GI #: 13569942
• NCBI GeneID: 81851
• NCBI Accession #: NP_112229.1
• NCBI GenBank Nucleotide #: NM_030967.2
• UniProt Accession #: Q07627; Q96S60; Q96S67; A6NC32
• Molecular Weight: 18,235 Da
• NCBI Official Full Name: keratin-associated protein 1-1
• NCBI Official Synonym Full Names: keratin associated protein 1-1
• NCBI Official Symbol: KRTAP1-1
• NCBI Official Synonym Symbols: HB2A; KAP1.1; KAP1.6; KAP1.7; KAP1.1A; KAP1.1B; KRTAP1A; hKAP1.7; KRTAP1.1
• NCBI Protein Information: keratin-associated protein 1-1; keratin associated protein 1A; keratin associated protein 1.7; keratin-associated protein 1.6; keratin-associated protein 1.7; high sulfur keratin-associated protein 1.1
• UniProt Protein Name: Keratin-associated protein 1-1
• Protein Family: Keratin-associated protein
• UniProt Gene Name: KRTAP1-1
• UniProt Synonym Gene Names: B2A; KAP1.1; KAP1.6; KAP1.7; KRTAP1.1
• UniProt Entry Name: KRA11_HUMAN

NCBI Description

This protein is a member of the keratin-associated protein (KAP) family. The KAP proteins form a matrix of keratin intermediate filaments which contribute to the structure of hair fibers. KAP family members appear to have unique, family-specific amino- and carboxyl-terminal regions and are subdivided into three multi-gene families according to amino acid composition: the high sulfur, the ultrahigh sulfur, and the high tyrosine/glycine KAPs. This protein is a member of the high sulfur KAP family and the gene is localized to a cluster of KAPs at 17q12-q21. [provided by RefSeq, Jul 2008]

Uniprot Description

KRTAP1-1: In the hair cortex, hair keratin intermediate filaments are embedded in an interfilamentous matrix, consisting of hair keratin-associated proteins (KRTAP), which are essential for the formation of a rigid and resistant hair shaft through their extensive disulfide bond cross-linking with abundant cysteine residues of hair keratins. The matrix proteins include the high- sulfur and high-glycine-tyrosine keratins. Belongs to the KRTAP type 1 family. Interacts with hair keratins

Chromosomal Location of Human Ortholog: 17q12

Cellular Component: keratin filament