Rabbit anti-Human EphA7 Polyclonal Antibody | anti-EphA7 antibody

EphA7, NT (EPHA7, EHK3, HEK11, Ephrin type-A receptor 7, EPH homology kinase 3, EPH-like kinase 11) (MaxLight 550)

Cat. Num. AAA6296026

• Gene Names: EPHA7; EHK3; HEK11
• Reactivity: Human
• Applications: Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Purified by Protein A/G Affinity Chromatography.
• Synonyms: EphA7; Polyclonal Antibody; NT (EPHA7; EHK3; HEK11; Ephrin type-A receptor 7; EPH homology kinase 3; EPH-like kinase 11) (MaxLight 550); EPHA7; EPH-like kinase 11; anti-EphA7 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human EphA7.
• Purity/Purification: Purified by Protein A/G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight550.

• Applicable Applications for anti-EphA7 antibody: Immunohistochemistry (IHC) Paraffin, Flow Cytometry (FC/FACS), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 11-41 in the N-terminal region of human EphA7, UniProt Accession #Q15375. Species sequence homology: mouse, rat
• Conjugate: MaxLight550
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue usingMBS647885, followed by peroxidase conjugated to the secondary antibody and DAB staining. This data demonstrates the use of this antibody for immunohistochemistry.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of A375 cells using MBS647885 (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-EphA7 antibody

Antibodies; Protein Kinases

References

1. Wilkinson, D.G., Nat Rev Neurosci 2(3):155-164 (2001). 2. Xu, Q., et al., Philos. Trans. R. Soc. Lond., B, Biol. Sci. 355(1399):993-1002 (2000). 3. Holder, N., et al., Development 126(10):2033-2044 (1999). 4. Zhou, R., Pharmacol. Ther. 77(3):151-181 (1998). 5. Fox, G.M., et al., Oncogene 10(5):897-905 (1995).

NCBI and Uniprot Product Information

• NCBI GI #: 4758282
• NCBI GeneID: 2045
• NCBI Accession #: NP_004431.1
• NCBI GenBank Nucleotide #: NM_004440.3
• UniProt Accession #: Q15375; Q59G40; Q5VTU0; Q8N368; Q9H124; A0AUX7; B2R8W1; B7ZLJ9; B7ZLK0
• Molecular Weight: 112,097 Da
• NCBI Official Full Name: ephrin type-A receptor 7
• NCBI Official Synonym Full Names: EPH receptor A7
• NCBI Official Symbol: EPHA7
• NCBI Official Synonym Symbols: EHK3; HEK11
• NCBI Protein Information: ephrin type-A receptor 7; EK11; EHK-3; EPH-like kinase 11; EPH homology kinase 3; Eph homology kinase-3; receptor protein-tyrosine kinase HEK11; tyrosine-protein kinase receptor EHK-3
• UniProt Protein Name: Ephrin type-A receptor 7
• Protein Family: Ephrin type-A receptor
• UniProt Gene Name: EPHA7
• UniProt Synonym Gene Names: EHK3; HEK11; EHK-3; EK11; hEK11
• UniProt Entry Name: EPHA7_HUMAN

NCBI Description

This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. [provided by RefSeq, Jul 2008]

Uniprot Description

EphA7: a widely-expressed receptor tyrosine kinase of the Eph family. Receptor for members of the ephrin-A family. Binds to ephrin-A1, -A2, -A3, -A4 and -A5. Contains 1 sterile alpha motif (SAM) and two fibronectin type III domains.

Protein type: Protein kinase, TK; Membrane protein, integral; Kinase, protein; EC 2.7.10.1; Protein kinase, tyrosine (receptor); TK group; Eph family

Chromosomal Location of Human Ortholog: 6q16.1

Cellular Component: postsynaptic membrane; cell soma; integral to plasma membrane; dendrite; plasma membrane; neuromuscular junction

Molecular Function: protein binding; ephrin receptor binding; chemorepellent activity; protein-tyrosine kinase activity; GPI-linked ephrin receptor activity; axon guidance receptor activity; ATP binding

Biological Process: axon guidance; regulation of cell-cell adhesion; peptidyl-tyrosine phosphorylation; branching morphogenesis of a nerve; regulation of protein amino acid autophosphorylation; positive regulation of neuron apoptosis; ephrin receptor signaling pathway; negative chemotaxis; brain development; regulation of caspase activity; phosphorylation; regulation of peptidyl-tyrosine phosphorylation; retinal ganglion cell axon guidance