Rabbit anti-Human CUGBP2 Polyclonal Antibody | anti-CUGBP2 antibody

CUGBP2, CT (CELF2, BRUNOL3, CUGBP2, ETR3, NAPOR, CUGBP Elav-like family member 2, Bruno-like protein 3, CUG triplet repeat RNA-binding protein 2, CUG-BP-and ETR-3-like factor 2, ELAV-type RNA-binding protein 3, Neuroblastoma apoptosis-related RNA-binding

Cat. Num. AAA6290034

• Gene Names: CELF2; ETR3; ETR-3; NAPOR; CUGBP2; BRUNOL3
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CUGBP2; Polyclonal Antibody; CT (CELF2; BRUNOL3; ETR3; NAPOR; CUGBP Elav-like family member 2; Bruno-like protein 3; CUG triplet repeat RNA-binding protein 2; CUG-BP-and ETR-3-like factor 2; ELAV-type RNA-binding protein 3; Neuroblastoma apoptosis-related RNA-binding; CELF2; Neuroblastoma apoptosis-related RNA-binding protein; RNA-binding protein BRUNOL-3; anti-CUGBP2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-CUGBP2 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: CUGBP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 480-508 amino acids from the C-terminal region of human CUGBP2.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(CUGBP2 Antibody (C-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the CUGBP2 antibody detected the CUGBP2 protein (arrow).)

Related Product Information for anti-CUGBP2 antibody

Members of the CELF/BRUNOL protein family contain two N-terminal RNA recognition motif (RRM) domains, one C-terminal RRM domain, and a divergent segment of 160-230 aa between the second and third RRM domains. Members of this protein family regulate pre-mRNA alternative splicing and may also be involved in mRNA editing, and translation. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq].

Product Categories/Family for anti-CUGBP2 antibody

Antibodies; Apoptosis

References

Shimada, M., et al. Hum. Genet. 128(4):433-441(2010). Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009). Anney, R.J., et al. Am. J. Med. Genet. B Neuropsychiatr. Genet. 147B (8), 1369-1378 (2008).

NCBI and Uniprot Product Information

• NCBI GI #: 68303645
• NCBI GeneID: 10659
• NCBI Accession #: NP_001020247.1; NP_001020248.1
• NCBI GenBank Nucleotide #: NM_001025076.2
• UniProt Accession #: O95319; Q7KYU4; Q8N499; Q92950; Q96NW9; Q96RQ5; Q96RQ6; Q9UL67; B7ZAN9
• Molecular Weight: 54,285 Da
• NCBI Official Full Name: CUGBP Elav-like family member 2 isoform 1
• NCBI Official Synonym Full Names: CUGBP, Elav-like family member 2
• NCBI Official Symbol: CELF2
• NCBI Official Synonym Symbols: ETR3; ETR-3; NAPOR; CUGBP2; BRUNOL3
• NCBI Protein Information: CUGBP Elav-like family member 2; CELF-2; CUG-BP2; bruno-like protein 3; RNA-binding protein BRUNOL-3; CUG-BP- and ETR-3-like factor 2; ELAV-type RNA-binding protein 3; CUG triplet repeat RNA-binding protein 2; neuroblastoma apoptosis-related RNA-binding p
• UniProt Protein Name: CUGBP Elav-like family member 2
• UniProt Gene Name: CELF2
• UniProt Synonym Gene Names: BRUNOL3; CUGBP2; ETR3; NAPOR; CELF-2; CUG-BP2; ETR-3; hNAPOR
• UniProt Entry Name: CELF2_HUMAN

Uniprot Description

CELF2: RNA-binding protein implicated in the regulation of several post-transcriptional events. Involved in pre-mRNA alternative splicing, mRNA translation and stability. Mediates exon inclusion and/or exclusion in pre-mRNA that are subject to tissue-specific and developmentally regulated alternative splicing. Specifically activates exon 5 inclusion of TNNT2 in embryonic, but not adult, skeletal muscle. Activates TNNT2 exon 5 inclusion by antagonizing the repressive effect of PTB. Acts as both an activator and repressor of a pair of coregulated exons: promotes inclusion of the smooth muscle (SM) exon but exclusion of the non-muscle (NM) exon in actinin pre-mRNAs. Promotes inclusion of exonS 21 and exclusion of exon 5 of the NMDA receptor R1 pre- mRNA. Involved in the apoB RNA editing activity. Increases COX2 mRNA stability and inhibits COX2 mRNA translation in epithelial cells after radiation injury. Modulates the cellular apoptosis program by regulating COX2-mediated prostaglandin E2 (PGE2) expression. Binds to (CUG)n triplet repeats in the 3'-UTR of transcripts such as DMPK. Binds to the muscle-specific splicing enhancer (MSE) intronic sites flanking the TNNT2 alternative exon 5. Binds preferentially to UG-rich sequences, in particular UG repeat and UGUU motifs. Binds to apoB mRNA, specifically to AU-rich sequences located immediatly upstream of the edited cytidine. Binds AU-rich sequences in the 3'-UTR of COX2 mRNA. Binds to an intronic RNA element responsible for the silencing of exon 21 splicing. Binds to (CUG)n repeats. Belongs to the CELF/BRUNOL family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding

Chromosomal Location of Human Ortholog: 10p13

Cellular Component: cytoplasm; nucleus

Molecular Function: RNA binding; nucleotide binding

Biological Process: RNA processing; mRNA processing; regulation of heart contraction