Rabbit anti-Human CENPH Polyclonal Antibody | anti-CENPH antibody

CENPH, ID (CENPH, ICEN35, Centromere protein H, Interphase centromere complex protein 35) (Biotin)

Cat. Num. AAA6285097

• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, ELISA, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CENPH; Polyclonal Antibody; ID (CENPH; ICEN35; Centromere protein H; Interphase centromere complex protein 35) (Biotin); Interphase centromere complex protein 35; anti-CENPH antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-CENPH antibody: Flow Cytometry (FC/FACS), ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; ELISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: CENPH antibody is generated from rabbits immunized with a KLH conjugated Synthetic peptide selected from the center region of human CENPH.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of CENPH Antibody (Center) in Hela cell line lysates (35ug/lane). CENPH (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human testis tissue reacted with CENPH Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(CENPH Antibody (Center) flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-CENPH antibody

Centromere and kinetochore proteins play a critical role in centromere structure, kinetochore formation, and sister chromatid separation. The protein encoded by this gene colocalizes with inner kinetochore plate proteins CENP-A and CENP-C in both interphase and metaphase. It localizes outside of centromeric heterochromatin, where CENP-B is localized, and inside the kinetochore corona, where CENP-E is localized during prometaphase. It is thought that this protein can bind to itself, as well as to CENP-A, CENP-B or CENP-C. Multimers of the protein localize constitutively to the inner kinetochore plate and play an important role in the organization and function of the active centromere-kinetochore complex.

Product Categories/Family for anti-CENPH antibody

Antibodies; Centromeres

NCBI and Uniprot Product Information

• NCBI GI #: 12597655
• NCBI GeneID: 64946
• NCBI Accession #: NP_075060.1
• NCBI GenBank Nucleotide #: NM_022909.3
• UniProt Accession #: Q9H3R5; A8K3Y1
• Molecular Weight: 28,481 Da
• NCBI Official Full Name: centromere protein H
• NCBI Official Synonym Full Names: centromere protein H
• NCBI Official Symbol: CENPH
• NCBI Protein Information: centromere protein H; CENP-H; kinetochore protein CENP-H; interphase centromere complex protein 35
• UniProt Protein Name: Centromere protein H
• Protein Family: Centromere protein
• UniProt Gene Name: CENPH
• UniProt Synonym Gene Names: ICEN35; CENP-H
• UniProt Entry Name: CENPH_HUMAN

NCBI Description

Centromere and kinetochore proteins play a critical role in centromere structure, kinetochore formation, and sister chromatid separation. The protein encoded by this gene colocalizes with inner kinetochore plate proteins CENP-A and CENP-C in both interphase and metaphase. It localizes outside of centromeric heterochromatin, where CENP-B is localized, and inside the kinetochore corona, where CENP-E is localized during prometaphase. It is thought that this protein can bind to itself, as well as to CENP-A, CENP-B or CENP-C. Multimers of the protein localize constitutively to the inner kinetochore plate and play an important role in the organization and function of the active centromere-kinetochore complex. [provided by RefSeq, Jul 2008]

Uniprot Description

CENPH: Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. Required for chromosome congression and efficiently align the chromosomes on a metaphase plate. Belongs to the centromere protein H family.

Protein type: Cell cycle regulation

Chromosomal Location of Human Ortholog: 5p15.2

Cellular Component: nucleoplasm; kinetochore; nucleus; cytosol

Molecular Function: kinetochore binding; protein binding

Biological Process: nucleosome assembly; kinetochore assembly; DNA replication-independent nucleosome assembly at centromere; mitotic cell cycle; kinetochore organization and biogenesis