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Western Blot (WB) (All lanes : Anti-MYBL2 Antibody (C-Term) at 1:8000 dilutionLane 1: CEM whole cell lysateLane 2: MCF-7 whole cell lysateLane 3: mouse testis lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 79 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Rabbit anti-Human, Mouse MYBL2 Polyclonal Antibody | anti-MYBL2 antibody

MYBL2 Antibody (C-Term)

Gene Names
MYBL2; BMYB; B-MYB
Reactivity
Human, Mouse
Applications
Western Blot
Synonyms
MYBL2; Polyclonal Antibody; MYBL2 Antibody (C-Term); Purified Rabbit Polyclonal Antibody (Pab); Myb-related protein B; B-Myb; Myb-like protein 2; BMYB; anti-MYBL2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
Ig
Specificity
This MYBL2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 540-571 amino acids from human MYBL2.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Length
676
Applicable Applications for anti-MYBL2 antibody
Western Blot (WB)
Application Notes
WB ~~ 1:8000
WB ~~ 1:2000
Antigen Source
Human
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(All lanes : Anti-MYBL2 Antibody (C-Term) at 1:8000 dilutionLane 1: CEM whole cell lysateLane 2: MCF-7 whole cell lysateLane 3: mouse testis lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 79 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB) (All lanes : Anti-MYBL2 Antibody (C-Term) at 1:8000 dilutionLane 1: CEM whole cell lysateLane 2: MCF-7 whole cell lysateLane 3: mouse testis lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 79 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB)

(All lanes : Anti-MYBL2 Antibody (C-Term) at 1:2000 dilutionLane 1: 293T/17 whole cell lysateLane 2: K562 whole cell lysateLane 3: Hela whole cell lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 79 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB) (All lanes : Anti-MYBL2 Antibody (C-Term) at 1:2000 dilutionLane 1: 293T/17 whole cell lysateLane 2: K562 whole cell lysateLane 3: Hela whole cell lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 79 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-MYBL2 antibody
Transcription factor involved in the regulation of cell survival, proliferation, and differentiation. Transactivates the expression of the CLU gene.
Product Categories/Family for anti-MYBL2 antibody
References
Nomura N.,et al.Nucleic Acids Res. 16:11075-11089(1988). Ota T.,et al.Nat. Genet. 36:40-45(2004). Totoki Y.,et al.Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases. Deloukas P.,et al.Nature 414:865-871(2001). Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
78764
NCBI Official Full Name
myb-related protein B isoform 2
NCBI Official Synonym Full Names
v-myb avian myeloblastosis viral oncogene homolog-like 2
NCBI Official Symbol
MYBL2
NCBI Official Synonym Symbols
BMYB; B-MYB
NCBI Protein Information
myb-related protein B
UniProt Protein Name
Myb-related protein B
Protein Family
UniProt Gene Name
MYBL2
UniProt Synonym Gene Names
BMYB; B-Myb
UniProt Entry Name
MYBB_HUMAN

NCBI Description

The protein encoded by this gene, a member of the MYB family of transcription factor genes, is a nuclear protein involved in cell cycle progression. The encoded protein is phosphorylated by cyclin A/cyclin-dependent kinase 2 during the S-phase of the cell cycle and possesses both activator and repressor activities. It has been shown to activate the cell division cycle 2, cyclin D1, and insulin-like growth factor-binding protein 5 genes. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2013]

Uniprot Description

B-Myb: a transcription factor involved in cell cycle progression. Phosphorylated by cyclin A/cyclin-dependent kinase 2 during the S-phase of the cell cycle and possesses both activator and repressor activities. It has been shown to activate the cell division cycle 2, cyclin D1, and insulin-like growth factor-binding protein 5 genes. Transcript variants may exist for this gene, but their full-length natures have not been determined.

Protein type: Motility/polarity/chemotaxis; Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 20q13.1

Cellular Component: nucleoplasm

Molecular Function: protein binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; regulation of cell cycle; positive regulation of transcription from RNA polymerase II promoter; mitotic cell cycle; G2/M transition of mitotic cell cycle

Research Articles on MYBL2

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Product Notes

The MYBL2 mybl2 (Catalog #AAA9215958) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MYBL2 Antibody (C-Term) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's MYBL2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB ~~ 1:8000 WB ~~ 1:2000. Researchers should empirically determine the suitability of the MYBL2 mybl2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MYBL2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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